Transplantation of a Mammary Stromal Cell Line into a Mammary Fat Pad: Development of the Site-Specific in Vivo Analysis System for Mammary Stromal Cells
Transplantation of a Mammary Stromal Cell Line into a Mammary Fat Pad: Development of the Site-Specific in Vivo Analysis System for Mammary Stromal Cells
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将乳腺基质细胞系移植到乳腺脂肪垫中：乳腺基质细胞位点特异性体内分析系统的开发
DOI：
10.1271/bbb.100773
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
T. Matsuda
中科院分区：
文献类型：
--
作者：
Hajime Nakatani;N. Aoki;D. Nadano;T. Matsuda
乳腺上皮组织和间质组织之间的相互作用被认为在乳腺组织发育中很重要。在这项研究中，我们开发了一种乳腺间质成纤维细胞系（MSF）的移植程序，以检查其在体内的生命。首先，我们建立了具有乳腺基质细胞特征的稳定表达lacZ的MSF细胞（lacZ/MSF）。然后将lacZ/MSF细胞移植到具有或不具有乳腺原代上皮类器官的同种小鼠的清除的乳腺脂肪垫中。移植瘤的全壁X-gal染色和胭脂红染色显示，在类器官移植后，大量未分化的lacZ/MSF细胞在乳腺上皮组织周围存活。这些结果表明，MSF细胞移植到乳腺脂肪垫是通过与原代乳腺类器官共移植实现的。最后，我们讨论了移植程序在乳腺间质组织发育和间质-上皮相互作用的体内研究中的应用。
The interaction between mammary epithelial and stromal tissue is considered to be important in breast tissue development. In this study, we developed a transplantation procedure for the mammary stromal fibroblastic cell line (MSF) to examine its life in vivo. First we established MSF cells which stably expressed lacZ (lacZ/MSF) and had characteristics of mammary stromal cells. The lacZ/MSF cells were then transplanted into a cleared mammary fat pad of syngenic mice with and without mammary primary epithelial organoids. Whole mount X-gal and carmine staining of the transplants revealed that a number of undifferentiated lacZ/MSF cells survived around the mammary epithelial tissue when transplanted with organoids. These results indicate that transplantation of MSF cells into mammary fat pad was accomplished by co-transplantation with primary mammary organoids. Finally, we discuss the application of transplantation procedure for in vivo studies of the mammary stromal tissue development and stromal-epithelial interactions.
DOI：
10.1016/j.bbrc.2004.09.053
发表时间：
2004-11
影响因子：
3.1
作者：
William C. Stewart;J. Baugh;Z. Floyd;J. Stephens
通讯作者：
William C. Stewart;J. Baugh;Z. Floyd;J. Stephens
DOI：
10.1073/pnas.81.12.3756
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DANIELSON, KG;OBORN, CJ;MEDINA, D
通讯作者：
MEDINA, D
影响因子：
4.1
作者：
Mei, BS;Zhao, L;Sul, HS
通讯作者：
Sul, HS