A rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia.
A rapid, low-cost, and microfluidic chip-based system for parallel identification of multiple pathogens related to clinical pneumonia.
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一种快速、低成本、基于微流控芯片的系统，用于并行识别与临床肺炎相关的多种病原体
DOI：
10.1038/s41598-017-06739-2
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发表时间：
2017-07-25
影响因子：
4.6
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Huang G;Huang Q;Xie L;Xiang G;Wang L;Xu H;Ma L;Luo X;Xin J;Zhou X;Jin X;Zhang L
设计了一种空气绝缘微流控芯片，用于将样品自动离心分配到24个测试细胞，使1.45-μL反应中多种临床肺炎相关病原体的并行鉴定能够在45 分钟内实现无交叉污染。还研制了一种集机械、共焦、光学、电子和软件功能于一体的便携式核酸分析仪，用于实时采集光学衍射限附近3 mm成像范围内的荧光数据，用于高灵敏的核酸扩增荧光检测。这种基于微流控芯片的便携式核酸分析仪可以检测到低丰度的核酸，只有10个拷贝。在一项盲法实验中，对肺炎支原体、金黄色葡萄球菌和甲氧西林耐药性进行了特异性鉴定。对229例临床患者痰标本进行检测，获得金黄色葡萄球菌。本系统与传统RT-PCR的总符合率为99.56%。对占0.44%不符合率的4个样本进行了基因测序，结果表明本系统报告的结果是正确的。这种新型的基于微流控芯片的检测系统具有成本低、快速、灵敏、特异的特点，并且具有较高的并行识别吞吐量，特别适用于资源有限的设施/区域和护理点检测。
An air-insulated microfluidic chip was designed for the automatic centrifugal distribution of samples to 24-test cells, enabling the parallel identification of multiple clinical pneumonia-related pathogens in 1.45-μL reactions without cross-contamination in 45 min. A portable nucleic acid analyzer that integrates mechanical, confocal optical, electronic, and software functions was also developed to collect fluorescence data in a Ø3 mm imaging field near the optical diffraction limit for highly sensitive fluorescence detection of nucleic acid amplification in real time. This microfluidic chip-based portable nucleic acid analyzer could detect low abundance nucleic acids present at as few as 10 copies. In a blinded experiment, specific identification ofMycoplasma pneumoniae,Staphylococcus aureus, and methicillin-resistantS. aureuswas achieved with 229 clinical patient sputum samples. The total coincidence rate of our system and traditional RT-PCR with an ABI 7500 was 99.56%. Four samples accounting for the 0.44% inconformity were retested by gene sequencing, revealing that our system reported the correct results. This novel microfluidic chip-based detection system is cost-effective, rapid, sensitive, specific, and has a relatively high throughput for parallel identification, which is especially suitable for resource-limited facilities/areas and point-of-care testing.
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影响因子：
2.2
作者：
Gotoh, Kensei;Nishimura, Naoko;Ozaki, Takao
通讯作者：
Ozaki, Takao
影响因子：
2.8
作者：
Ahmad, Farhan;Seyrig, Gregoire;Hashsham, Syed A.
通讯作者：
Hashsham, Syed A.
影响因子：
11.8
作者：
Liu, Catherine;Bayer, Arnold;Chambers, Henry F.
通讯作者：
Chambers, Henry F.
DOI：
10.1016/j.nano.2013.05.016
发表时间：
2013-11-01
影响因子：
5.4
作者：
Chang, Wen-Hsin;Yang, Sung-Yi;Lee, Gwo-Bin
通讯作者：
Lee, Gwo-Bin
影响因子：
9.4
作者：
Okafuji, T;Yoshida, N;Nakayama, T
通讯作者：
Nakayama, T