In vitro enzymatic biodegradation of adhesive resin in the hybrid layer.
In vitro enzymatic biodegradation of adhesive resin in the hybrid layer.
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DOI：
10.1002/jbm.a.32689
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发表时间：
2010-07
影响因子：
4.9
通讯作者：
Armstrong, Steven R.
中科院分区：
文献类型：
--
作者：
Zou, Yuan;Jessop, Julie L. P.;Armstrong, Steven R.
粘合剂渗透到脱矿牙本质表面及其随后的转化对粘合树脂-牙本质粘结的寿命至关重要。通过拉曼光谱监测老化样本混合层内粘合剂浓度的变化，研究树脂-牙本质粘结的耐久性。在光聚合后24小时和在三种介质中的一种中储存24周后，测量树脂-牙本质样本的混合层中的Bis-GMA和HEMA的绝对摩尔浓度：人工唾液（SAL）、含有胆固醇酯酶的SAL攻击树脂（EST）和含有细菌胶原酶的SAL攻击胶原（COL）。在24小时储存时，这些组中的Bis-GMA和HEMA摩尔浓度没有显著差异;然而，浓度从粘合剂树脂到混合层的中间降低。在SAL中老化后，任何树脂-牙本质位置的浓度保持不变。在混合层，浓度显着降低，随着老化的COL和EST的下降趋势。虽然内源性基质金属蛋白酶和唾液酯酶显示出潜在的酶促生物降解作用，但这种方法也可能被证明是对新化学物质和未来改善树脂-牙本质粘结性能的方法的有价值的评估。
Penetration of adhesives into the demineralized dentin surface and their subsequent conversion are critically important to longevity of the adhesive resin-dentin bond. The durability of the resin-dentin bond is investigated by monitoring the change of adhesive concentration within the hybrid layer of aged specimens using Raman spectroscopy. Absolute molar concentrations of Bis-GMA and HEMA were measured across the hybrid layer of resin-dentin specimens 24 h after photopolymerization and after 24-wk storage in one of three media: artificial saliva (SAL), SAL containing cholesterol esterase to attack resin (EST), and SAL containing bacterial collagenase to attack collagen (COL). No significant difference among these groups for both Bis-GMA and HEMA molar concentrations at 24-h storage was found; however, concentrations decreased from the adhesive resin to the middle of the hybrid layer. Concentrations remained unchanged at any resin-dentin position after aging in SAL. In the hybrid layer, concentrations significantly decreased with aging in COL and tended to decrease in EST. While showing potential enzymatic biodegradative effects of endogenous matrix metalloproteinases and salivary esterases, this methodology may also prove to be a valuable assessment of new chemistries and future approaches to improve resin-dentin bond performance.
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影响因子：
5
作者：
Phrukkanon, S;Burrow, MF;Tyas, MJ
通讯作者：
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影响因子：
4.9
作者：
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通讯作者：
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DOI：
10.1002/jbm.b.10560
发表时间：
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