QTL analyses of temporal and intensity components of home-cage activity in KJR and C57BL/6J strains.
QTL analyses of temporal and intensity components of home-cage activity in KJR and C57BL/6J strains.
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DOI：
10.1186/1471-2156-10-40
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发表时间：
2009-07-29
期刊：
影响因子：
2.9
通讯作者：
Koide T
中科院分区：
文献类型：
--
作者：
Umemori J;Nishi A;Lionikas A;Sakaguchi T;Kuriki S;Blizard DA;Koide T
各种小鼠品系在自发活动中表现出多样性，这与重要的遗传贡献一致。迄今为止，许多研究将自发的家庭笼活动定义为在测试期间的总距离或总活动计数。然而，自发活动实际上是“时间”和“强度”元素的组合，类似于“速度”。在这里，我们报告了自发活动的不同组成部分的数量性状位点，这是解剖潜在遗传机制的重要一步。在雌性小鼠适应后的总家笼活性（THA）分析中，KJR菌株的活性高于C57BL/6J （B6）。在本研究中，THA被划分为两个组成部分：活动时间（AT）是THA的“时间因素”指标，活动时间期间的平均活动（AA）是THA的“强度”指标。B6xKJR F2雌性小鼠的相关分析表明，AA是THA的主要组成部分，而AA和AT的相关程度较低。为了探究活性差异的遗传基础，我们对THA及其组分、AT和AA进行了数量性状位点（QTL）分析。鉴定出3个影响家笼不同组分活性变异的显著QTL，两个连锁QTL Hylaq1和Hylaq2位于Chr 2， Hylaq3位于Chr 10。这些QTL的染色体位置先前与运动活动（Chr 2）或野外活动（Chr 10）有关。结果表明，Hylaq1影响AT、Hylaq2和AA，而Hylaq3同时与AA和AT相关。通过这项研究，我们发现在3天的时间内，总家庭笼活动的变化受到活动时间和活动强度的变化的影响。后两个变量是家庭笼活动的不同组成部分，只有部分重叠的遗传结构。
A variety of mouse strains exhibit diversity in spontaneous activity consistent with an important genetic contribution. To date, many studies have defined spontaneous home-cage activity as total distance or total counts of activity within a test period. However, spontaneous activity is, in fact, a composite of elements of 'temporal' and 'intensity' that is similar to 'velocity'. Here, we report on quantitative trait loci for different components of spontaneous activity, an important step towards dissection of the underlying genetic mechanisms. In the analysis of total home-cage activity (THA) after habituation in female mice, KJR strain exhibit higher activity than C57BL/6J (B6). In this study, THA was partitioned into two components: active time (AT) was an index of the 'temporal element' of THA, average activity during active time (AA) was an index of 'intensity'. Correlation analysis using B6xKJR F2 female mice indicated that AA is a major component of THA, whereas AA and AT were associated to a lesser degree. To explore the genetic basis of the activity differences, we conducted quantitative trait loci (QTL) analysis on data of THA and its components, AT and AA. Three significant QTL affecting variation of different components of home cage activity were identified, two linked QTL Hylaq1 and Hylaq2 on Chr 2, and Hylaq3 on Chr 10. Chromosomal positions of these QTL were previously implicated in locomotor activity (Chr 2) or open-field ambulation (Chr 10). The results indicated that Hylaq1 influences AT, Hylaq2, AA, while Hylaq3 is associated with both AA and AT. Through this study, we found that variation in total home cage activity over a 3 day period is affected by variation in active time and intensity of activity. The latter two variables are distinct components of home cage activity with only partially overlapping genetic architecture.
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影响因子：
--
作者：
HENDLEY, ED;OHLSSON, WG
通讯作者：
OHLSSON, WG
影响因子：
2.6
作者：
Koyner, J;Demarest, K;Hitzemann, R
通讯作者：
Hitzemann, R
影响因子：
2.5
作者：
Koide, T;Moriwaki, K;Shiroishi, T
通讯作者：
Shiroishi, T
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2.5
作者：
Kas, M. J. H.;Malsen, J. G. de Mooij-van;de Graan, P. N. E.
通讯作者：
de Graan, P. N. E.
影响因子：
2.5
作者：
Hitzemann, R;Cipp, L;McCaughran, J
通讯作者：
McCaughran, J