Rapid changes in histone deacetylases and inflammatory gene expression in expert meditators.
Rapid changes in histone deacetylases and inflammatory gene expression in expert meditators.
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DOI：
10.1016/j.psyneuen.2013.11.004
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发表时间：
2014-02
影响因子：
3.7
通讯作者：
Davidson, Richard J.
中科院分区：
文献类型：
--
作者：
Kaliman, Perla;Alvarez-Lopez, Maria Jesus;Cosin-Tomas, Marta;Rosenkranz, Melissa A.;Lutz, Antoine;Davidson, Richard J.
越来越多的研究表明，正念冥想可以改变神经、行为和生化过程。然而，这种临床相关效应的机制仍然难以捉摸。在这里，我们探讨了在有经验的受试者(n=19)中进行一天的集中正念冥想对外周血单核细胞(PBMC)中昼夜节律基因、染色质调节基因和炎症基因表达的影响。同时，我们分析了一组没有冥想经验的控制组，他们在相同的环境中从事休闲活动(n=21)。在干预前(T1)和干预后(T2)(T2-T1=8h)采集所有受试者的PBMC，并采用定制通路聚焦的定量-实时聚合酶链式反应分析基因表达。两组都接受了特里尔社会压力测试(TSST)。核心时钟基因在基线(T1)时的表达在不同组之间是相似的，冥想者的节律性不受密集练习一天的影响。同样，我们发现所有表观遗传调节酶和炎症基因分析在两组中显示出相似的基础表达水平。相反，在短暂的干预后，我们检测到与对照组相比，冥想者组蛋白脱乙酰酶基因(HDAC2、3和9)的表达减少，组蛋白的整体修饰发生变化(H4ac；H3K4me3)，促炎基因(RIPK2和COX2)的表达减少。我们发现RIPK2和HDAC2基因的表达与两组皮质醇恢复到TSST的速度有关。HDAC和炎症通路的调节可能代表了正念干预治疗潜力的一些潜在机制。我们的发现为进一步评估治疗慢性炎症的冥想策略的未来研究奠定了基础。
A growing body of research shows that mindfulness meditation can alter neural, behavioral and biochemical processes. However, the mechanisms responsible for such clinically relevant effects remain elusive. Here we explored the impact of a day of intensive practice of mindfulness meditation in experienced subjects (n= 19) on the expression of circadian, chromatin modulatory and inflammatory genes in peripheral blood mononuclear cells (PBMCs). In parallel, we analyzed a control group of subjects with no meditation experience who engaged in leisure activities in the same environment (n= 21). PBMCs from all participants were obtained before (t1) and after (t2) the intervention (t2-t1= 8 hours) and gene expression was analyzed using custom pathway focused quantitative-real time PCR assays. Both groups were also presented with the Trier Social Stress Test (TSST). Core clock gene expression at baseline (t1) was similar between groups and their rhythmicity was not influenced in meditators by the intensive day of practice. Similarly, we found that all the epigenetic regulatory enzymes and inflammatory genes analyzed exhibited similar basal expression levels in the two groups. In contrast, after the brief intervention we detected reduced expression of histone deacetylase genes (HDAC2, 3 and 9), alterations in global modification of histones (H4ac; H3K4me3) and decreased expression of pro-inflammatory genes (RIPK2 and COX2) in meditators compared with controls. We found that the expression of RIPK2 and HDAC2 genes was associated with a faster cortisol recovery to the TSST in both groups. The regulation of HDACs and inflammatory pathways may represent some of the mechanisms underlying the therapeutic potential of mindfulness-based interventions. Our findings set the foundation for future studies to further assess meditation strategies for the treatment of chronic inflammatory conditions.
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影响因子：
4.7
作者：
Chandramohan, Yalini;Droste, Susanne K.;Reul, Johannes M. H. M.
通讯作者：
Reul, Johannes M. H. M.
影响因子：
5.2
作者：
Epel E;Daubenmier J;Moskowitz JT;Folkman S;Blackburn E
通讯作者：
Blackburn E
影响因子：
7.4
作者：
Brown, Christopher A.;Jones, Anthony K. P.
通讯作者：
Jones, Anthony K. P.
影响因子：
29.4
作者：
de Zoeten EF;Wang L;Sai H;Dillmann WH;Hancock WW
通讯作者：
Hancock WW
影响因子：
1.9
作者：
Kavcic P;Rojc B;Dolenc-Groselj L;Claustrat B;Fujs K;Poljak M
通讯作者：
Poljak M