The acrosomal protein SP-10 (Acrv1) is an ideal marker for staging of the cycle of seminiferous epithelium in the mouse.
The acrosomal protein SP-10 (Acrv1) is an ideal marker for staging of the cycle of seminiferous epithelium in the mouse.
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DOI：
10.1002/mrd.22358
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发表时间：
2014-10
影响因子：
2.5
通讯作者：
Reddi, Prabhakara P.
中科院分区：
文献类型：
--
作者：
Osuru, Hari Prasad;Monroe, Jennifer E.;Chebolu, Apoorv P.;Akamune, Joycelyn;Pramoonjago, Patcharin;Ranpura, Sandeep A.;Reddi, Prabhakara P.
对小鼠精子发生的研究需要准确识别生精上皮的周期阶段。阶段指的是生殖细胞类型在特定发育阶段的独特联系，如睾丸横切面所示。然而，筹划的过程是一项艰巨的任务。小鼠生精管有12个发育阶段。阶段通常是根据精子细胞顶体发育的形态来确定的。尽管顶体的特征在超微结构图像中得到了很好的记录，但缺乏一种能够在光学显微镜下突出顶体形状细微差异的试剂。在这里，我们证明了一种针对小鼠顶体蛋白SP-10的多克隆抗体对于分期鉴定非常有用。免疫组织化学显示，抗SP-10抗体对精子细胞的顶体高度特异，因为上皮组织中没有其他类型的细胞显示免疫反应。在较低的放大倍数下，顶体的大体形状和免疫组织化学染色强度的增加可以作为识别阶段1-12的指南。在较高的放大倍数下，可以明确地识别特征的形态特征，例如与核表面接触的顶体部分是圆形的(阶段3)、扁平的(阶段4)还是弯曲的(阶段6)。总体而言，我们提出的证据表明，SP-10是一个有用的标记，可以用来判断生精上皮的周期。抗SP-10抗体在不同的固定剂、石蜡包埋和冰冻切片上都能很好地工作；它也被证明对鉴定突变小鼠的生精缺陷很有用。
The study of spermatogenesis in the mouse requires accurate identification of the cycle stage of seminiferous epithelium. A stage refers to the unique association of germ-cell types at a particular phase of development, as seen in a testicular cross section. The process of staging, however, is a daunting task. There are 12 stages represented in the mouse seminiferous tubule. Stages are typically identified on the basis of the morphology of the developing acrosome of spermatids. Although the characteristic features of the acrosome are well-documented in ultrastructure images, a reagent that can highlight the subtle differences in acrosome shape under the light microscope is lacking. Here we demonstrate that a polyclonal antibody raised against the mouse acrosomal protein SP-10 is extremely useful for stage identification. Immunohistochemistry showed that the anti-SP-10 antibody is highly specific for the acrosome of spermatids, as no other cell type in the epithelium showed immunoreactivity. At lower magnification, the gross shape of the acrosome and the increasing intensity of immunostaining served as a guide for the identification of stages 1–12. At higher magnification, characteristic morphological features –such as whether the part of the acrosome that contacts the nuclear surface is round (stage 3) or flat (stage 4) or curved (stage 6)– could be identified unambiguously. Overall, we present evidence that SP-10 is a useful marker for staging the cycle of a seminiferous epithelium. The anti-SP-10 antibody works well in different fixatives, on paraffin-embedded as well as cryosections; it has also been shown to be useful for characterizing spermatogenic defects in mutant mice.
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影响因子：
4.8
作者：
Lalmansingh, Avin S.;Urekar, Craig J.;Reddi, Prabhakara P.
通讯作者：
Reddi, Prabhakara P.
DOI：
10.1083/jcb.43.3.575
发表时间：
1969-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Langreth SG
通讯作者：
Langreth SG
影响因子：
3.6
作者：
Kim, KS;Foster, JA;Gerton, GL
通讯作者：
Gerton, GL
影响因子：
4.6
作者：
Pan, JY;Goodheart, M;Wang, PJ
通讯作者：
Wang, PJ
影响因子：
3.6
作者：
HERR, JC;KLOTZ, K;FLICKINGER, CJ
通讯作者：
FLICKINGER, CJ