IncFV plasmid pED208: Sequence analysis and evidence for translocation of maintenance/leading region proteins through diverse type IV secretion systems.
IncFV plasmid pED208: Sequence analysis and evidence for translocation of maintenance/leading region proteins through diverse type IV secretion systems.
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DOI：
10.1016/j.plasmid.2022.102652
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发表时间：
2022-09
期刊：
影响因子：
2.6
通讯作者：
Christie, Peter J.
中科院分区：
文献类型：
--
作者：
Al Mamun, Abu Amar M.;Kissoon, Kimberly;Kishida, Kouhei;Shropshire, William C.;Hanson, Blake;Christie, Peter J.
关键词：
两个遗传学上远相关的IncF质粒F和pED 208是研究F样IV型分泌系统（T4 SSFs）和F皮利的机制和结构的重要模型。在这里，我们展示了pED 208序列，并将其与F和pUMNF 18进行比较，pUMNF 18是NCBI数据库中与pED 208最接近的匹配。正如预期的那样，三个货物区域的基因含量变化很大，尽管维持/前导区（MLR）和转移（Tra）区域也携带对质粒稳定性、传播和宿主范围具有预测调节作用的新基因或基序。通过使用Cre易位重组酶测定（CRAfT），我们最近报道了携带pED 208的供体通过T4 SSF在细胞间易位MLR的几种产物（帕拉、ParB 1、ParB 2、SSB、PsiB、PsiA）。在这里，我们通过报告携带pED 208的供体在接合过程中易位10个额外的MLR蛋白来扩展这些发现。与此相反，两个F质粒编码的毒素成分的毒素抗毒素（TA）模块，CcdB和SrnB，没有易位在可检测的水平通过T4 SSF。值得注意的是，大多数或所有pED 208编码的MLR蛋白和CcdB和SrnB分别通过IncN和IncP质粒pKM 101和RP 4编码的异源T4 SS易位。总之，我们的序列分析强调了F质粒超家族的基因组多样性，我们的实验数据证明了蛋白质易位的接合机器的混杂性质。我们的研究结果提出了有趣的问题T4 SS易位信号的性质和共轭蛋白质转移的生物和进化后果。
Two phylogenetically distantly-related IncF plasmids, F and pED208, serve as important models for mechanistic and structural studies of F-like type IV secretion systems (T4SSFs) and F pili. Here, we present the pED208 sequence and compare it to F and pUMNF18, the closest match to pED208 in the NCBI database. As expected, gene content of the three cargo regions varies extensively, although the maintenance/leading regions (MLRs) and transfer (Tra) regions also carry novel genes or motifs with predicted modulatory effects on plasmid stability, dissemination and host range. By use of a Cre recombinase assay for translocation (CRAfT), we recently reported that pED208-carrying donors translocate several products of the MLR (ParA, ParB1, ParB2, SSB, PsiB, PsiA) intercellularly through the T4SSF. Here, we extend these findings by reporting that pED208-carrying donors translocate 10 additional MLR proteins during conjugation. In contrast, two F plasmid-encoded toxin components of toxin-antitoxin (TA) modules, CcdB and SrnB, were not translocated at detectable levels through the T4SSF. Remarkably, most or all of the pED208-encoded MLR proteins and CcdB and SrnB were translocated through heterologous T4SSs encoded by IncN and IncP plasmids pKM101 and RP4, respectively. Together, our sequence analyses underscore the genomic diversity of the F plasmid superfamily, and our experimental data demonstrate the promiscuous nature of conjugation machines for protein translocation. Our findings raise intriguing questions about the nature of T4SS translocation signals and of the biological and evolutionary consequences of conjugative protein transfer.
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影响因子：
2.8
作者：
Bates, S;Roscoe, RA;Wilkins, BM
通讯作者：
Wilkins, BM
影响因子：
64.5
作者：
Costa TRD;Ilangovan A;Ukleja M;Redzej A;Santini JM;Smith TK;Egelman EH;Waksman G
通讯作者：
Waksman G
影响因子：
4.5
作者：
Baharoglu Z;Bikard D;Mazel D
通讯作者：
Mazel D
影响因子：
3.6
作者：
Lang S;Kirchberger PC;Gruber CJ;Redzej A;Raffl S;Zellnig G;Zangger K;Zechner EL
通讯作者：
Zechner EL
影响因子：
3.2
作者：
ARMSTRONG, GD;FROST, LS;PARANCHYCH, W
通讯作者：
PARANCHYCH, W