Hyperactivation is sufficient to release porcine sperm from immobilized oviduct glycans.
Hyperactivation is sufficient to release porcine sperm from immobilized oviduct glycans.
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DOI：
10.1038/s41598-022-10390-x
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发表时间：
2022-04-19
影响因子：
4.6
通讯作者：
Miller, David J.
中科院分区：
文献类型：
--
作者：
Sharif, Momal;Hickl, Vincent;Juarez, Gabriel;Di, Xingjian;Kerns, Karl;Sutovsky, Peter;Bovin, Nicolai;Miller, David J.
受精精子通过粘附于输卵管上皮上的特异性聚糖而被保留，从而在精子从储库中释放之前形成储库，因此受精可以随之发生。获能精子失去了对输卵管上皮的亲和力，但对精子释放很重要的获能成分尚不确定。获能的一个重要关联是超活化运动的发展。超激活的特征在于具有高搏动幅度的不对称鞭毛搏动。我们测试了全型不对称运动的发展是否足以从固定的输卵管聚糖中释放精子。精子超活化由四种不同的化合物诱导，细胞渗透性cAMP类似物（cBiMPS），CatSper激活剂（4-氨基吡啶和普鲁卡因），和内源性类固醇（孕酮）。使用标准分析（CASA）和高速视频显微镜直接可视化，我们首先证实，所有四种化合物诱导超活化。随后，使精子与固定的输卵管聚糖结合，并加入化合物或溶剂对照。所有化合物均导致精子从固定化聚糖中释放，表明超活化足以从输卵管细胞和固定化聚糖中释放精子。非基因组孕酮受体和CatSper的药理学抑制减少了输卵管聚糖的精子释放。抑制泛素-蛋白酶体系统（UPS）的蛋白水解活性，参与精子获能的调节，减少精子释放响应所有超活化诱导剂。总之，诱导精子超活化足以诱导精子从固定的输卵管聚糖中释放，释放依赖于CatSper和UPS。
Fertilizing sperm are retained by adhesion to specific glycans on the epithelium of the oviduct forming a reservoir before sperm are released from the reservoir so fertilization can ensue. Capacitated sperm lose affinity for the oviduct epithelium but the components of capacitation that are important for sperm release are uncertain. One important correlate of capacitation is the development of hyperactivated motility. Hyperactivation is characterized by asymmetrical flagellar beating with high beat amplitude. We tested whether the development of full-type asymmetrical motility was sufficient to release sperm from immobilized oviduct glycans. Sperm hyperactivation was induced by four different compounds, a cell-permeable cAMP analog (cBiMPS), CatSper activators (4-aminopyridine and procaine), and an endogenous steroid (progesterone). Using standard analysis (CASA) and direct visualization with high-speed video microscopy, we first confirmed that all four compounds induced hyperactivation. Subsequently, sperm were allowed to bind to immobilized oviduct glycans, and compounds or vehicle controls were added. All compounds caused sperm release from immobilized glycans, demonstrating that hyperactivation was sufficient to release sperm from oviduct cells and immobilized glycans. Pharmacological inhibition of the non-genomic progesterone receptor and CatSper diminished sperm release from oviduct glycans. Inhibition of the proteolytic activities of the ubiquitin–proteasome system (UPS), implicated in the regulation of sperm capacitation, diminished sperm release in response to all hyperactivation inducers. In summary, induction of sperm hyperactivation was sufficient to induce sperm release from immobilized oviduct glycans and release was dependent on CatSper and the UPS.
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影响因子：
64.5
作者：
Chung JJ;Shim SH;Everley RA;Gygi SP;Zhuang X;Clapham DE
通讯作者：
Clapham DE
影响因子：
1.9
作者：
Ho, Katharine;Wolff, Collin A.;Suarez, Susan S.
通讯作者：
Suarez, Susan S.
影响因子：
2
作者：
Curtis, M. P.;Kirkman-Brown, J. C.;Gaffney, E. A.
通讯作者：
Gaffney, E. A.
DOI：
10.1073/pnas.2536658100
发表时间：
2003-12-09
影响因子：
11.1
作者：
Carlson, AE;Westenbroek, RE;Babcock, DF
通讯作者：
Babcock, DF
影响因子：
2.8
作者：
Daigneault, Bradford W.;McNamara, Kelli A.;Miller, David J.
通讯作者：
Miller, David J.