The segregation of Escherichia coli minichromosomes constructed in vivo by recombineering.
The segregation of Escherichia coli minichromosomes constructed in vivo by recombineering.
复制标题
DOI：
10.1016/j.plasmid.2012.01.002
复制
发表时间：
2012-03
期刊：
影响因子：
2.6
通讯作者：
Austin S
中科院分区：
文献类型：
--
作者：
Sawitzke JA;Youngren B;Thomason LC;Baker T;Sengupta M;Court D;Austin S
含有复制起点oriC的染色体的环状区域可以保持为自主微型染色体oriC质粒。我们表明，oriC质粒含有精确的，预先确定的染色体片段，可以通过一个简单的体内重组技术。我们产生了两个这样的质粒携带荧光标记。将这些转移到oriC染色体拷贝附近具有不同荧光标记的受体菌株中。因此，oriC质粒和染色体起源的命运可以独立地在活细胞中通过荧光显微镜观察。与以前的报告相比，我们发现，有一个强烈的趋势oriC质粒拷贝积累在细胞中心作为一个单一或双焦点在平面上的细胞分裂。这不是简单地由于从类核空间排除，而是似乎是一个特定的识别和保留的质粒的一些中央定位的细胞位点。
Circularized regions of the chromosome containing the origin of replication, oriC, can be maintained as autonomous minichromosomes, oriC plasmids. We show that oriC plasmids containing precise, pre-determined segments of the chromosome can be generated by a simple in vivo recombineering technique. We generated two such plasmids carrying fluorescent markers. These were transferred to a recipient strain with a different fluorescent marker near the chromosomal copy of oriC. Thus the fates of the oriC plasmid and chromosomal origins could be followed independently in living cells by fluorescence microscopy. In contrast to a previous report, we show that there is a strong tendency of oriC plasmid copies to accumulate at the cell center as a single or double focus at the plane of cell division. This is not simply due to exclusion from the nucleoid space but rather appears to be a specific recognition and retention of the plasmid by some central-located cell site.
登录
查看更多内容
影响因子：
3.6
作者：
Nielsen, Henrik J.;Li, Yongfang;Austin, Stuart
通讯作者：
Austin, Stuart
影响因子：
3.2
作者：
Nielsen, Henrik J.;Youngren, Brenda;Austin, Stuart
通讯作者：
Austin, Stuart
DOI：
10.1073/pnas.83.14.5101
发表时间：
1986-07-01
影响因子：
11.1
作者：
LEONARD, AC;HELMSTETTER, CE
通讯作者：
HELMSTETTER, CE
影响因子：
3.6
作者：
Niki, H;Hiraga, S
通讯作者：
Hiraga, S
DOI：
10.1101/sqb.1979.043.01.018
发表时间：
1979-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
VONMEYENBURG, K;HANSEN, FG;MESSER, W
通讯作者：
MESSER, W