Examination of human osteoarchaeological remains as a feasible source of polar and apolar metabolites to study past conditions.

Examination of human osteoarchaeological remains as a feasible source of polar and apolar metabolites to study past conditions.
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DOI:
10.1038/s41598-023-27401-0
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发表时间:
2023-01-13
期刊:
影响因子:
4.6
通讯作者:
--
中科院分区:
综合性期刊3区
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--
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代谢组学是一种现代工具,有助于我们了解生物体的分子变化。考古学是考古学的一个分支,它使用现代分析工具探索不同的考古材料。人类骨考古材料是考古背景下经常发现的,有可能提供关于以前人类种群的信息,这可以照亮我们当前的状况。利用一组包含不同骨骼元素和骨骼结构的样本,我们首次探索了从骨考古材料中提取代谢物的可能性。本文提出了一种利用超高效液相色谱联用高分辨率质谱法提取和测量极性和低极性/极代谢物的方案,以测量经反相和亲水性相互作用的液相色谱柱分离的分子。获得了分子信息,表明骨考古材料是代谢组学研究的分子信息的可行来源。
Metabolomics is a modern tool that aids in our understanding of the molecular changes in organisms. Archaeological science is a branch of archaeology that explores different archaeological materials using modern analytical tools. Human osteoarchaeological material are a frequent finding in archaeological contexts and have the potential to offer information about previous human populations, which can be illuminating about our current condition. Using a set of samples comprising different skeletal elements and bone structures, here we explore for the first time the possibility of extracting metabolites from osteoarchaeological material. Here, a protocol for extraction and measurement of extracted polar and less-polar/apolar metabolites by ultra-high performance liquid chromatography hyphenated to high resolution mass spectrometry is presented to measure the molecules separated after a reversed phase and hydrophilic interaction liquid chromatography column. Molecular information was obtained, showing that osteoarchaeological material is a viable source of molecular information for metabolomic studies.
骨研究中的代谢组学。
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