Correlation between steroid levels in follicular fluid and hormone synthesis related substances in its exosomes and embryo quality in patients with polycystic ovary syndrome.
Correlation between steroid levels in follicular fluid and hormone synthesis related substances in its exosomes and embryo quality in patients with polycystic ovary syndrome.
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多囊卵巢综合征患者卵泡液类固醇水平及其外泌体激素合成相关物质与胚胎质量的关系。
DOI：
10.1186/s12958-021-00749-6
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发表时间：
2021-05-17
期刊：
影响因子：
--
通讯作者：
Guo W
中科院分区：
文献类型：
--
作者：
Yu L;Liu M;Wang Z;Liu T;Liu S;Wang B;Pan B;Dong X;Guo W
多囊卵巢综合征(PCOS)是一种临床表现多样、病因复杂的内分泌代谢紊乱疾病。卵泡液是卵泡发育的复杂微环境。然而，激素浓度与PCOS发病机制之间的关系尚不清楚。用LC-MS/MS法检测了10例PCOS患者和10例男性因素不育症患者外周血中20种类固醇激素水平，以探讨其与PCOS的关系。同时，采用定量聚合酶链式反应(QPCR)技术检测了类固醇合成途径中的核心酶基因的表达水平。与正常组比较，多囊卵巢综合征组FF中雌三醇(p <； 0.0 1)、雌二醇(p <； 0.0 5)和孕酮(p <； 0.0 1)水平显著升高，孕酮(p <； 0.0 5)水平明显降低。外切体细胞色素P11A、19A和HSD17B2基因表达水平的升高伴随着激素水平的变化。相关分析表明，CYP11A和HSD17B2的表达水平与优质胚胎率和胚胎发育率呈负相关。我们的结果提示，卵泡液中雌激素和孕烯醇酮水平的升高可能影响PCOS患者卵泡的发育，其机制部分与FFExosome中HSD17B1、CYP19A1和Cyp11a1表达的变化有关。网上版载有补充材料，可在10.1186/s12958-021-00749-6查阅。
Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disorder with various manifestations and complex etiology. Follicular fluid (FF) serves as the complex microenvironment for follicular development. However, the correlation between the concentration of steroid in FF and the pathogenesis of PCOS is still unclear. Twenty steroid levels in FF from ten patients with PCOS and ten women with male-factor infertility undergoing in vitro fertilization were tested by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in order to explore their possibly correlation with PCOS. Meanwhile, the mRNA levels of core enzymes in steroid synthesis pathway from exosomes of FF were also detected by qPCR. The estriol (p < 0.01), estradiol (p < 0.05) and prenenolone (p < 0.01) levels in FF of PCOS group were significantly increased, compared to the normal group, and the progesterone levels (p < 0.05) were decreased in PCOS group. Increased mRNA levels of CYP11A, CYP19A and HSD17B2 of exosomes were accompanied by the hormonal changes in FF. Correlation analysis showed that mRNA levels of CYP11A and HSD17B2 were negatively correlated with percent of top-quality embryos and rate of embryos develop to blastocyst. Our results suggest that increased levels of estrogen and pregnenolone in follicular fluid may affect follicle development in PCOS patients, and the mechanism is partially related to HSD17B1, CYP19A1 and CYP11A1 expression change in FF exosomes. The online version contains supplementary material available at 10.1186/s12958-021-00749-6.
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