Localization of zip1 and zip4 mRNA in the adult rat brain.
Localization of zip1 and zip4 mRNA in the adult rat brain.
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DOI：
10.1002/jnr.22144
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发表时间：
2009-11-01
影响因子：
4.2
通讯作者：
Bressler, Joseph
中科院分区：
文献类型：
--
作者：
Belloni-Olivi, Luisa;Marshall, Cathleen;Laal, Bachchu;Andrews, Glenn K.;Bressler, Joseph
在成年小鼠的大脑中检查了锌转运蛋白 Slc39a 家族的两个成员（zip1 和 zip4）的定位。 Zip1 在神经元细胞体密度高的大脑区域高度富集，包括海马体、丘脑和角周皮层。 Zip1也在胼胝体和前连合等连合纤维束中表达，但在内囊和外囊中表达甚少。此外，在刺伤后 14 天检查的静息星形胶质细胞和反应性星形胶质细胞中检测到极少量的 zip1 mRNA。在第三脑室和侧脑室衬里的室管膜细胞以及脉络丛的上皮细胞中检测到 Zip1 mRNA。有趣的是，在脉络丛中检测到 zip4 mRNA，但在室管膜细胞或其他神经元件中未检测到。大脑毛细血管中也检测到了 Zip4 mRNA，但未检测到 zip1 mRNA。在表达受 zip4 启动子调控的绿色荧光蛋白的 zip4 敲除杂合子中，在脑毛细血管中检测到绿色荧光蛋白。由于 zip4 水平受膳食锌的调节，我们的研究表明大脑具有适应锌状态变化的潜力。
The localization of two members of the Slc39a (zip1 and zip4) family of zinc transporters was examined in the brains of adult mice. Zip1 was highly enriched in brain regions with high densities of neuronal cell bodies, including the hippocampus, thalamus, and perifontal cortex. Zip1 was also expressed in commissural fiber tracts such as the corpus callosum and anterior commissure, but little was found in the internal and external capsules. Also, very low amounts of zip1 mRNA were detected in resting astrocytes and reactive astrocytes that were examined at 14 days after inflicting a stab wound. Zip1 mRNA was detected in ependymal cells lining the third and lateral ventricles and epithelium cells in the choroid plexus. Interestingly, zip4 mRNA was detected in the choroid plexus but not in the ependymal cells or other neural elements. Zip4 mRNA was also detected in brain capillaries, but zip1 mRNA was not. In zip4 knockout heterozygotes that express green fluorescent protein regulated by the zip4 promoter, green fluorescent protein was detected in brain capillaries. Because zip4 levels are regulated by dietary Zn, our studies suggest that the brain has the potential of adapting to changes in Zn status.
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