Glycogen metabolism is required for optimal cyanobacterial growth in the rapid light-dark cycle of low-Earth orbit.
Glycogen metabolism is required for optimal cyanobacterial growth in the rapid light-dark cycle of low-Earth orbit.
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DOI：
10.1016/j.lssr.2022.11.001
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发表时间：
2023-03
影响因子：
2.5
通讯作者：
Golden, James W.
中科院分区：
文献类型：
--
作者：
Bishe, Bryan;Golden, Susan S.;Golden, James W.
一些用于人类太空任务的生物再生生命支持系统的设计包含蓝细菌，用于去除二氧化碳，产生氧气和处理废水，以及提供营养来源。在这项研究中，我们研究了低地球轨道的短光暗（LD）周期对藻类和蓝藻生长的影响，近似于国际空间站的条件，大约每90分钟绕地球轨道运行一次。我们发现绿藻在正常的12 h光照：12 h黑暗（12 h:12 h LD）和45 ':45 ' LD循环下的生长相似。三种不同的蓝藻菌株不仅能够在45 ‘:45 ’的短周期内生长，而且实际上比12 h:12 h的LD周期生长得更好。我们发现45 ':45 ' LD周期不影响长聚球菌的内源性24小时昼夜节律。利用密集的随机条形码转座子突变体文库，我们确定了在45 ':45 ' LD循环下，失去对S. elongatus生长有害的基因。其中包括参与糖原代谢和磷酸戊糖氧化途径的几个基因。值得注意的是，45 ':45 ' LD循环不会影响携带生物昼夜节律振荡器或时钟输入和输出调节途径突变的菌株的适应性。总的来说，这项研究表明蓝藻的培养可以在低地球轨道的自然阳光下生长，并强调了在模式生物中进行功能基因组研究的效用，以更好地了解与太空旅行相关的条件下的关键生物过程。
Some designs for bioregenerative life support systems to enable human space missions incorporate cyanobacteria for removal of carbon dioxide, generation of oxygen, and treatment of wastewater, as well as providing a source of nutrition. In this study, we examined the effects of the short light-dark (LD) cycle of low-Earth orbit on algal and cyanobacterial growth, approximating conditions on the International Space Station, which orbits Earth roughly every 90 min. We found that growth of green algae was similar in both normal 12 h light:12 h dark (12 h:12 h LD) and 45′:45′ LD cycles. Three diverse strains of cyanobacteria were not only capable of growth in short 45′:45′ LD cycles, but actually grew better than in 12 h:12 h LD cycles. We showed that 45′:45′ LD cycles do not affect the endogenous 24 h circadian rhythms of Synechococcus elongatus. Using a dense library of randomly barcoded transposon mutants, we identified genes whose loss is detrimental for the growth of S. elongatus under 45′:45′ LD cycles. These include several genes involved in glycogen metabolism and the oxidative pentose phosphate pathway. Notably, 45′:45′ LD cycles did not affect the fitness of strains that carry mutations in the biological circadian oscillator or the clock input and output regulatory pathways. Overall, this study shows that cultures of cyanobacteria could be grown under natural sunlight of low-Earth orbit and highlights the utility of a functional genomic study in a model organism to better understand key biological processes in conditions that are relevant to space travel.
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影响因子：
2.8
作者：
Inoue K;Araki T;Endo M
通讯作者：
Endo M
影响因子：
8.8
作者：
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影响因子：
64.8
作者：
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通讯作者：
Deutschbauer, Adam M.
DOI：
10.1073/pnas.1519220112
发表时间：
2015-12-01
影响因子：
11.1
作者：
Rubin, Benjamin E.;Wetmore, Kelly M.;Golden, Susan S.
通讯作者：
Golden, Susan S.
影响因子：
2.6
作者：
Hendrickx, L;De Wever, H;Mergeay, M
通讯作者：
Mergeay, M