Quantitative proteomic analysis of age-related subventricular zone proteins associated with neurodegenerative disease.
Quantitative proteomic analysis of age-related subventricular zone proteins associated with neurodegenerative disease.
复制标题
与神经退行性疾病相关的年龄相关室下区蛋白的定量蛋白质组学分析。
DOI：
10.1038/srep37443
复制
发表时间：
2016-11-18
影响因子：
4.6
通讯作者：
Xu J
中科院分区：
文献类型：
--
作者：
Wang X;Dong C;Sun L;Zhu L;Sun C;Ma R;Ning K;Lu B;Zhang J;Xu J
衰老的特征是成人组织功能的进行性下降，这可能导致神经退行性疾病。然而，关于脑室下区(SVZ)的蛋白质变化与神经退行性疾病之间的相关性，随着年龄的增长，人们知之甚少。在本研究中，神经干细胞取自出生后7d、1 m和12 m龄小鼠。随着年龄的增长，神经干细胞的SA-β-GAL活性增强，SVZ区的增殖和池大小减小，并与炎性趋化因子和细胞因子的升高有关。此外，定量蛋白质组学和独创性通路分析被用来评估与年龄相关的蛋白质及其功能的显著变化。一些下调的蛋白如DPYSL2、TPI1、ALDH和UCHL1被发现在神经系统疾病中起关键作用，而PSMA1、PSMA3、PSMC2、PSMD11和UCHL1在蛋白质稳态中起关键作用。综上所述，我们对SVZ神经发生衰老相关下降背后的细胞和分子过程提供了有价值的见解，以便及早发现基因表达的差异和神经疾病的潜在风险，这有助于疾病的预防。
Aging is characterized by a progressive decline in the function of adult tissues which can lead to neurodegenerative disorders. However, little is known about the correlation between protein changes in the subventricular zone (SVZ) and neurodegenerative diseases with age. In the present study, neural stem cells (NSCs) were derived from the SVZ on postnatal 7 d, 1 m, and 12 m-old mice. With age, NSCs exhibited increased SA-β-gal activity and decreased proliferation and pool size in the SVZ zone, and were associated with elevated inflammatory chemokines and cytokines. Furthermore, quantitative proteomics and ingenuity pathway analysis were used to evaluate the significant age-related alterations in proteins and their functions. Some downregulated proteins such as DPYSL2, TPI1, ALDH, and UCHL1 were found to play critical roles in the neurological disease and PSMA1, PSMA3, PSMC2, PSMD11, and UCHL1 in protein homeostasis. Taken together, we have provided valuable insight into the cellular and molecular processes that underlie aging-associated declines in SVZ neurogenesis for the early detection of differences in gene expression and the potential risk of neurological disease, which is beneficial in the prevention of the diseases.
登录
查看更多内容
影响因子：
64.5
作者：
López-Otín C;Blasco MA;Partridge L;Serrano M;Kroemer G
通讯作者：
Kroemer G
影响因子：
4.5
作者：
Chondrogianni, N;Fragoulis, EG;Gonos, ES
通讯作者：
Gonos, ES
DOI：
10.1073/pnas.92.20.9363
发表时间：
1995-09-26
影响因子：
11.1
作者：
DIMRI, GP;LEE, XH;CAMPISI, J
通讯作者：
CAMPISI, J
DOI：
10.1083/jcb.201010131
发表时间：
2011-04-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Liu L;Rando TA
通讯作者：
Rando TA
影响因子：
64.8
作者：
Molofsky, Anna V.;Slutsky, Shalom G.;Morrison, Sean J.
通讯作者：
Morrison, Sean J.