A mathematical model for understanding synergistic regulations and paradoxical feedbacks in the shoot apical meristem.
A mathematical model for understanding synergistic regulations and paradoxical feedbacks in the shoot apical meristem.
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DOI：
10.1016/j.csbj.2020.11.017
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发表时间：
2020
影响因子：
6
通讯作者：
Hong T
中科院分区：
文献类型：
--
作者：
Liu Z;Shpak ED;Hong T
茎尖分生组织（SAM）是植物茎中的初级干细胞生态位. SAM中的干细胞由复杂的调控网络控制，包括WUSCHEL（WUS）和CLAVATA 3（CLV 3）之间的负反馈。最近，我们确定了一组信号，表皮图案化因子样（EPFL）蛋白，产生于外周区域，对SAM的稳态很重要。在这里，我们提出了一个数学模型的SAM监管网络。该模型揭示了SAM以协同方式使用EPFL和信号（例如来自中间的HAIRY MERISTEM）来约束WUS和CLV 3。我们发现，WUS和CLV 3之间相互关联的负反馈和正反馈确保了在SAM中稳定的WUS表达，当面临扰动时，正反馈回路还在顶-基底方向上维持含有WUSon和CLV 3 on细胞的不同细胞群。此外，参数的系统扰动揭示了多个图案化特征的优化之间的权衡。我们的研究结果提供了一个整体的看法，SAM图案在多个维度的监管。他们深入了解了拟南芥如何整合来自横向和顶基轴的信号来控制SAM模式，并揭示了可能对理解干细胞生态位调控网络广泛有用的设计原理。
The shoot apical meristem (SAM) is the primary stem cell niche in plant shoots. Stem cells in the SAM are controlled by an intricate regulatory network, including negative feedback between WUSCHEL (WUS) and CLAVATA3 (CLV3). Recently, we identified a group of signals, Epidermal Patterning Factor-Like (EPFL) proteins, that are produced at the peripheral region and are important for SAM homeostasis. Here, we present a mathematical model for the SAM regulatory network. The model revealed that the SAM uses EPFL and signals such as HAIRY MERISTEM from the middle in a synergistic manner to constrain both WUS and CLV3. We found that interconnected negative and positive feedbacks between WUS and CLV3 ensure stable WUS expression in the SAM when facing perturbations, and the positive feedback loop also maintains distinct cell populations containing WUSon and CLV3on cells in the apical-basal direction. Furthermore, systematic perturbations of the parameters revealed a tradeoff between optimizations of multiple patterning features. Our results provide a holistic view of the regulation of SAM patterning in multiple dimensions. They give insights into how Arabidopsis integrates signals from lateral and apical-basal axes to control the SAM patterning, and they shed light into design principles that may be widely useful for understanding regulatory networks of stem cell niche.
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影响因子：
56.9
作者：
Fletcher, LC;Brand, U;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
DOI：
10.1016/j.biosystems.2018.07.006
发表时间：
2018-09
期刊：
Bio Systems
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