Effect of salvianolic acid A and C compatibility on inflammatory cytokines in rats with unilateral ureteral obstruction
Effect of salvianolic acid A and C compatibility on inflammatory cytokines in rats with unilateral ureteral obstruction
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丹酚酸A、C配伍对单侧输尿管梗阻大鼠炎症细胞因子的影响
DOI：
10.1016/s0254-6272(15)30140-0
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发表时间：
2015-10
影响因子：
2.6
通讯作者：
Zhao Renjie
中科院分区：
文献类型：
--
作者：
Li Jun;Gu Tong;Fu Xu;Zhao Renjie
目的探讨丹参素A和丹参素C组分分子对单侧输尿管梗阻（UUO）大鼠炎症细胞因子表达的影响。正常组、模型组、丹参酮A组、丹参酮C组、丹参酮A组和丹参酮C组。正常组作为对照组，其余各组均建立UUO模型。对照组给予自由进食和饮水，其余各组给予相应药物治疗，疗程均为2周。末次给药后，检测尿β2-微球蛋白（β2-MG）和N-乙酰-β-D-氨基葡萄糖苷酶（NAG）水平。24 h后处死所有大鼠，分析血清中肌酐（Cr）和血尿素氮（BUN）水平。取出大鼠肾脏，采用实时荧光定量逆转录聚合酶链反应（RT-PCR）测定CCL 5和CXCL 10炎性细胞因子mRNA的表达。结果丹参酮A、丹参酮C治疗组大鼠血清Cr、尿NAG水平均显著低于模型组（P均<0. 05）;各治疗组尿β2-MG水平均显著低于模型组（均P < 0.05）。与模型组比较，病理改变及胶原沉积均有不同程度的改善（均P < 0.05）。结论丹参素A和丹参素C是药物配伍的组成分子，在一定程度上可保护UUO大鼠肾功能，改善肾小管功能和肾脏病理。这种改善可能与UUO大鼠肾脏中炎性细胞因子CCL 5和CXCL 10分泌的减少有关。
ObjectiveTo investigate the effect of salvianolic acid A and C component molecules, which are involved in drug compatibility, on inflammatory cytokine expression that affects human chemokine ligand 5 (CCL5) and chemokine ligand 10 (CXCL10) levels in rats with unilateral ureteral obstruction (UUO).MethodsFifty Sprague Dawley rats were randomly divided into five groups: normal, model, salvianolic acid A, salvianolic acid C and salvianolic acid A and C groups. The normal group was used as the control, and the other groups of rats had a UUO model established. The control group had free access to food and water, and the other groups received the corresponding drugs for 2 weeks. After the last administration, urine β2-microglobulin (β2-MG) and N-acetyl-β-D-glucosaminidase (NAG) levels were analyzed. After 24 h, all rats were sacrificed and the serum was analyzed for creatinine (Cr) and blood urea nitrogen (BUN) levels. Rat kidneys were removed, and CCL5 and CXCL10 inflammatory cytokine mRNA expression was measured using real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR). Kidney fibrosis was observed using hematoxylin-eosin (HE) staining and Masson trichrome staining.ResultsIn the salvianolic acid A and salvianolic acid C treatment groups, serum Cr and urine NAG levels were significantly lower than in the model group (bothP< 0.05). In all treatment groups, urine β2-MG levels were significantly lower than in the model group (allP< 0.05). Compared with model group, the pathological changes and collagen deposition improved to varying degrees (bothP< 0.05). CCL5 and CXCL10 mRNA expression decreased to different degrees compared with the model group (bothP< 0.05).ConclusionSalvianolic acid A and C are component molecules of drug compatibility, and they may protect renal function and improve tubular function and renal pathology to a certain degree in UUO. This improvement may be related to a reduction in inflammatory cytokines CCL5 and CXCL10 secretion in the UUO rat kidney.
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DOI：
10.1111/j.1523-1755.2005.09423.x
发表时间：
2005-04
期刊：
Kidney international. Supplement
影响因子：
--
作者：
G. Zheng;Yiping Wang;D. Mahajan;X. Qin;Ying Wang;Yuanmin Wang;S. Alexander;D. Harris
通讯作者：
G. Zheng;Yiping Wang;D. Mahajan;X. Qin;Ying Wang;Yuanmin Wang;S. Alexander;D. Harris
影响因子：
--
作者：
Hu Zhuo-wei
通讯作者：
Hu Zhuo-wei
DOI：
--
发表时间：
2010-11
期刊：
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
影响因子：
--
作者：
Hai-ying Lu;Juan Zhou;Min Lu;Yu-min Liu;Fei Wang;Min Lin;Yue Zhang
通讯作者：
Hai-ying Lu;Juan Zhou;Min Lu;Yu-min Liu;Fei Wang;Min Lin;Yue Zhang
DOI：
10.1097/01.asn.0000102479.92582.43
发表时间：
2004-01-01
影响因子：
13.6
作者：
Nightingale, J;Patel, S;Zhang, Z
通讯作者：
Zhang, Z
影响因子：
3.4
作者：
A. Negri
通讯作者：
A. Negri