Reduced expression of the LRP16 gene in mouse insulinoma (MIN6) cells exerts multiple effects on insulin content, proliferation and apoptosis
Reduced expression of the LRP16 gene in mouse insulinoma (MIN6) cells exerts multiple effects on insulin content, proliferation and apoptosis
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小鼠胰岛素瘤 (MIN6) 细胞中 LRP16 基因表达减少对胰岛素含量、增殖和凋亡产生多重影响
DOI：
10.1007/s11596-012-0034-6
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发表时间：
2012
影响因子：
--
通讯作者：
Mu, Yiming
中科院分区：
文献类型：
--
作者：
Li, Xiaojin;Xue, Bing;Wang, Xuan;Sun, Lianqing;Zhang, Tingting;Qu, Ling;Zou, Xiaoman;Mu, Yiming
本研究评估了白血病相关蛋白16 （LRP16）对小鼠胰岛素瘤（MIN6）细胞胰腺功能调节的影响。通过shRNA干扰策略获得LRP16表达下调的细胞。用放射免疫法检测胰岛素含量和葡萄糖刺激胰岛素分泌（GSIS）。Western blotting检测蛋白表达。葡萄糖刺激下PDX-1的亚细胞定位通过免疫细胞化学测定。流式细胞术检测细胞增殖和凋亡情况。结果表明，LRP16通过控制胰岛素和胰岛素转录因子的表达来调节MIN6细胞中的胰岛素含量。LRP16基因沉默导致MIN6细胞增殖减少，凋亡增加。葡萄糖刺激下丝氨酸-473 Akt磷酸化和PDX-1向细胞核定位的观察表明，LRP16是介导MIN6细胞中Akt信号传导的成分。这些结果表明LRP16在维持胰腺β细胞功能中起关键作用，并可能有助于我们了解雌激素对胰腺β细胞功能的保护作用。
This study assessed the effects of leukemia-related protein 16 (LRP16) on the regulation of pancreatic functions in mouse insulinoma (MIN6) cells. Cells with down-regulated expression of LRP16 were obtained by a shRNA interference strategy. Insulin content and glucose-stimulated insulin secretion (GSIS) were examined by radioimmunoassay. Western blotting was applied to detect protein expression. Glucose-stimulated sub-cellular localization of PDX-1 was immunocytochemically determined. Cell proliferation and apoptosis were detected by flow cytometry. Our results showed that LRP16 regulated insulin content in MIN6 cells by controlling expression of insulin and insulin transcription factors. LRP16 gene silence in MIN6 cells led to reduced cell proliferation and increased apoptosis. The observation of phosphorylation of serine-473 Akt and the localization of PDX-1 to the nucleus under glucose-stimulation exhibited that LRP16 was a component mediating Akt signaling in MIN6 cells. These results suggest that LRP16 plays a key role in maintaining pancreatic β-cell functions and may help us to understand the protective effects of estrogen on the functions of pancreatic β-cells.
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影响因子：
--
作者：
Suwattanee Kooptiwut;Namoiy Semprasert;S. Chearskul
通讯作者：
Suwattanee Kooptiwut;Namoiy Semprasert;S. Chearskul
雌激素调节的 LRP16 与雌激素受体 α 相互作用并增强受体的转录活性
影响因子：
3.9
作者：
<strong>韩为东</strong>
通讯作者：
<strong>韩为东</strong>
雌激素受体ERα调节胰腺胰岛素含量。
影响因子：
3.7
作者：
Alonso-Magdalena, Paloma;Ropero, Ana B.;Carrera, M. Pilar;Cederroth, Christopher R.;Baquie, Mathurin;Gauthier, Benoit R.;Nef, Serge;Stefani, Enrico;Nadal, Angel
通讯作者：
Nadal, Angel
影响因子：
3.9
作者：
Zhenhua Ren;C. Zou;Huijun Ji;Y. A. Zhang
通讯作者：
Zhenhua Ren;C. Zou;Huijun Ji;Y. A. Zhang
影响因子：
7.7
作者：
Zhiyong Wang;H. Gleichmann
通讯作者：
Zhiyong Wang;H. Gleichmann