Multiplexed Ultrasound Imaging Using Spectral Analysis on Gas Vesicles.
Multiplexed Ultrasound Imaging Using Spectral Analysis on Gas Vesicles.
复制标题
使用气囊泡的光谱分析多路复用超声成像。
DOI：
10.1002/adhm.202200568
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发表时间：
2022-09
影响因子：
10
通讯作者：
Yoon, Sangpil
中科院分区：
文献类型：
--
作者：
Kim, Sangnam;Zhang, Siyuan;Yoon, Sangpil
关键词：
目前超声成像技术的进步与下一代造影剂如气泡的结合使生物组织的可视化具有时空精度的革命性变化。在光学方面，由于荧光蛋白的多路成像能力，使我们能够了解生物系统中的分子和细胞功能。在这里，我们研究了一组气泡，使用中带拟合光谱成像来实现多路超声成像，以唯一地可视化不同类型的固定气泡的位置。中带拟合谱成像技术表明，在琼脂糖凝胶模型中，固定的簇状气泡可以有效地定位并与非簇状气泡区分开来，并在体外小鼠肝脏标本中显示了三维血管结构。小鼠巨噬细胞作为聚集和非聚集气泡和多路信标的载体，通过发出不同的光谱信号来报告细胞的空间位置。重建二维中带拟合谱图像，并通过比较预测具有聚集和非聚集气泡的细胞的存在的预定过滤器，根据中带拟合值对这些图像中的像素进行分类。这种伪染色方案清晰地区分了两类细胞的位置，就像荧光显微镜中的伪彩色图像一样。我们提出了一种通用的方法来实现多路超声成像，以可视化嵌入聚集和非聚集气泡(GV)的细胞的位置。我们利用聚集的和非聚集的GV被小鼠巨噬细胞吞噬，在超声激励下发出明显的回声光谱。中带拟合谱成像将高频超声换能器在毫米深度获取的回波频谱与微米分辨率区分开来。
Current advances in ultrasound imaging techniques combined with the next generation contrast agents such as gas vesicles revolutionize the visualization of biological tissues with spatiotemporal precision. In optics, fluorescent proteins enable us to understand molecular and cellular functions in biological systems due to their multiplexed imaging capability. Here, we investigate a panel of gas vesicles using mid-band fit spectral imaging to realize multiplexed ultrasound imaging to uniquely visualize locations of different types of stationary gas vesicles. Mid-band fit spectral imaging technique demonstrates that stationary clustered gas vesicles are efficiently localized and distinguished from unclustered gas vesicles in agarose gel phantom and three-dimensional vessel structures are visualized in ex vivo mouse liver specimens. Mouse macrophages serve as carriers of clustered and unclustered gas vesicles and multiplexing beacons to report cells’ spatial locations by emitting distinct spectral signals. Two-dimensional mid-band fit spectral images are reconstructed, and pixels in these images are classified depending on mid-band fit values by comparing predetermined filters that predict the existence of cells with clustered and unclustered gas vesicles. This pseudo-coloring scheme clearly distinguishes the locations of two classes of cells like pseudo-color images in fluorescence microscopy. We propose a general approach to realize multiplexed ultrasound imaging to visualize locations of cells embedding clustered and unclustered gas vesicles (GV). We utilize clustered and unclustered GVs phagocytosed by mouse macrophages that emit distinct echo spectrum under ultrasound excitation. Mid-band fit spectral imaging differentiates echo spectrum acquired by high frequency ultrasonic transducers at millimeter depth with micrometer resolution.
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影响因子：
41.2
作者：
Lu GJ;Farhadi A;Szablowski JO;Lee-Gosselin A;Barnes SR;Lakshmanan A;Bourdeau RW;Shapiro MG
通讯作者：
Shapiro MG
影响因子：
17.1
作者：
Lakshmanan A;Farhadi A;Nety SP;Lee-Gosselin A;Bourdeau RW;Maresca D;Shapiro MG
通讯作者：
Shapiro MG
DOI：
10.1109/tbme.2021.3070487
发表时间：
2021-11
期刊：
IEEE transactions on bio-medical engineering
影响因子：
--
作者：
Kim J;Wang Q;Zhang S;Yoon S
通讯作者：
Yoon S
影响因子：
56.9
作者：
CHALFIE, M;TU, Y;PRASHER, DC
通讯作者：
PRASHER, DC
影响因子：
2.4
作者：
Han, Aiguo;Abuhabsah, Rami;O'Brien, William D., Jr.
通讯作者：
O'Brien, William D., Jr.