Supported lipid bilayer platforms to probe cell mechanobiology.
Supported lipid bilayer platforms to probe cell mechanobiology.
复制标题
DOI：
10.1016/j.bbamem.2017.05.005
复制
发表时间：
2017-09
期刊：
影响因子：
--
通讯作者：
Salaita K
中科院分区：
文献类型：
--
作者：
Glazier R;Salaita K
哺乳动物和细菌细胞通过机械转导过程感知并施加机械力，从而相互转换生化信号和物理信号。这在接触依赖性信号传导中尤其重要，其中配体-受体结合发生在细胞-细胞或细胞-ECM 连接处。由于发生在这些专门的连接处，参与接触依赖性信号传导的受体经历寡聚化并与细胞骨架偶联，作为其信号传导机制的一部分。虽然我们测量和绘制细胞连接内生化信号传导的能力在过去几十年中有所进步，但物理线索仍然难以在空间和时间上绘制地图。最近，支持的脂质双层（SLB）技术已成为一种灵活的平台，用于测量和操纵膜受体机械转导，使人们能够模拟细胞与细胞的连接。改变脂质成分和底层底物可调节双层流动性，脂质和配体的微米和纳米图案在空间上控制受体的定位和聚集。 SLB 内的金属网格线图案引入了限制脂质流动性并引入机械阻力的围栏。在这里，我们回顾了基本的 SLB 力学以及如何将 SLB 设计为用于机械转导研究的可调细胞基质。最后，我们强调这项工作对理解细胞粘附的生物物理机制的影响。
Mammalian and bacterial cells sense and exert mechanical forces through the process of mechanotransduction, which interconverts biochemical and physical signals. This is especially important in contact-dependent signaling, where ligand-receptor binding occurs at cell-cell or cell-ECM junctions. By virtue of occurring within these specialized junctions, receptors engaged in contact-dependent signaling undergo oligomerization and coupling with the cytoskeleton as part of their signaling mechanisms. While our ability to measure and map biochemical signaling within cell junctions has advanced over the past decades, physical cues remain difficult to map in space and time. Recently, supported lipid bilayer (SLB) technologies have emerged as a flexible platform to measure and manipulate membrane receptor mechanotransduction, allowing one to mimic cell-cell junctions. Changing the lipid composition and underlying substrate tunes bilayer fluidity, and lipid and ligand micro- and nano-patterning spatially control positioning and clustering of receptors. Patterning metal gridlines within SLBs introduces corrals that confine lipid mobility and introduce mechanical resistance. Here we review fundamental SLB mechanics and how SLBs can be engineered as tunable cell substrates for mechanotransduction studies. Finally, we highlight the impact of this work in understanding the biophysical mechanisms of cell adhesion.
登录
查看更多内容
影响因子：
3.4
作者：
DeMond, Andrew L.;Mossman, Kaspar D.;Groves, Jay T.
通讯作者：
Groves, Jay T.
影响因子：
10.8
作者：
Deeg J;Axmann M;Matic J;Liapis A;Depoil D;Afrose J;Curado S;Dustin ML;Spatz JP
通讯作者：
Spatz JP
影响因子：
15
作者：
Chang, Yuan;Liu, Zheng;Salaita, Khalid
通讯作者：
Salaita, Khalid
影响因子：
3.4
作者：
Eghiaian, Frederic;Rigato, Annafrancesca;Scheuring, Simon
通讯作者：
Scheuring, Simon
影响因子：
56.9
作者：
EDIDIN, M;KUO, SC;SHEETZ, MP
通讯作者：
SHEETZ, MP