Down-regulation of ABCG2 and ABCB4 transporters in the placenta of rats exposed to cadmium.
Down-regulation of ABCG2 and ABCB4 transporters in the placenta of rats exposed to cadmium.
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镉暴露大鼠胎盘中 ABCG2 和 ABCB4 转运蛋白的下调
DOI：
10.18632/oncotarget.9415
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发表时间：
2016-06-21
期刊：
影响因子：
--
通讯作者：
Ding Z
中科院分区：
文献类型：
--
作者：
Liu L;Zhou L;Hu S;Zhou S;Deng Y;Dong M;Huang J;Zeng Y;Chen X;Zhao N;Li H;Ding Z
镉（Cd）是一种母体和发育毒性物质，其透过胎盘屏障的能力较弱.然而，其潜在机制仍不清楚。为了深入了解胎盘中与镉毒性相关的蛋白质分子并探讨它们在镉转运中的作用，使用Sprague-Dawley大鼠进行了生殖动物实验。我们进行了胎盘蛋白质组分析的差异凝胶电泳（DIGE）结合基质辅助激光解吸/电离飞行时间串联质谱（MALDI-TOF/TOF MS）。DIGE分析确定了15个差异表达的蛋白质点，与对照组大鼠相比，镉处理大鼠胎盘中的变化大于1.5倍。基于蛋白的表达模式和生物学功能，我们选择ABCG 2和ABCB 4转运蛋白进行进一步分析。Western blot分析显示，镉暴露可下调胎盘中ABCG 2和ABCB 4的表达。镉暴露与ABCG 2和ABCB 4蛋白表达呈负剂量反应关系。上述结果提示，ABCG 2和ABCB 4转运蛋白的下调可能通过调节镉通过胎盘的转运而影响镉对胎儿的体内毒性作用。
As a maternal and developmental toxicant, cadmium (Cd) possesses weak penetrability through the placental barrier. However, the underlying mechanism remains unclear. To gain insight into the protein molecules associated with Cd toxicity in placenta and explore their roles in Cd transportation, a reproductive animal experiment was carried out using Sprague-Dawley rats. We performed proteomic analysis of the placenta by Difference Gel Electrophoresis (DIGE) combined with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Tandem Mass Spectroscopy (MALDI-TOF/TOF MS). The DIGE assay identified 15 protein spots that were differentially expressed with a greater than 1.5-fold change in placenta of Cd-treated rats compared to the control rats. Based on the expression patterns and biological functions of the proteins, we selected the ABCG2 and ABCB4 transporter proteins for further analysis. Western blot analysis showed that Cd exposure could down-regulate the expression of ABCG2 and ABCB4 in the placenta. There was a negative dose-response relationship between Cd exposure and the expression of ABCG2 or ABCB4 protein. These results indicated that down-regulation of ABCG2 and ABCB4 transporters may regulate Cd across through placenta and thus affect the in vivo toxic effect of Cd to fetus.
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影响因子：
2
作者：
Benitez, M. A.;Mendez-Armenta, M.;Rios, C.
通讯作者：
Rios, C.
影响因子：
6.8
作者：
Cazan, Alfy Morales;Klerks, Paul L.
通讯作者：
Klerks, Paul L.
影响因子：
8.3
作者：
Kantola, M;Purkunen, R;Vartiainen, T
通讯作者：
Vartiainen, T
影响因子：
2.3
作者：
Bircsak KM;Aleksunes LM
通讯作者：
Aleksunes LM
DOI：
10.1073/pnas.95.26.15665
发表时间：
1998-12-22
影响因子：
11.1
作者：
Doyle, LA;Yang, WD;Ross, DD
通讯作者：
Ross, DD