Evaluation of the comet assay for assessing the dose-response relationship of DNA damage induced by ionizing radiation.
Evaluation of the comet assay for assessing the dose-response relationship of DNA damage induced by ionizing radiation.
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DOI：
10.3390/ijms141122449
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发表时间：
2013-11-14
影响因子：
5.6
通讯作者：
Liu Q
中科院分区：
文献类型：
--
作者：
Wang Y;Xu C;Du LQ;Cao J;Liu JX;Su X;Zhao H;Fan FY;Wang B;Katsube T;Fan SJ;Liu Q
剂量和时间响应曲线相结合，以评估辐射生物剂量学的彗星试验的潜力。应用中性彗星试验检测γ射线照射后淋巴细胞DNA双链断裂。彗星试验显示，在照射后不同时间，DNA双链断裂存在明显的剂量-反应关系（p < 0.001）。照射后72 h内，也存在时间-效应关系（P < 0.001）。人淋巴细胞DNA双链断裂和DNA修复的体外曲线具有良好的模型性，将两条曲线结合后可得到光滑的三维平面模型。
Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relationship was also found within 72 h after irradiation (p < 0.001). The curves for DNA double-strand breaks and DNA repair in vitro of human lymphocytes presented a nice model, and a smooth, three-dimensional plane model was obtained when the two curves were combined.
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DOI：
10.1186/1748-717x-7-61
发表时间：
2012-04-20
期刊：
Radiation oncology (London, England)
影响因子：
--
作者：
Seidel C;Lautenschläger C;Dunst J;Müller AC
通讯作者：
Müller AC
影响因子：
2
作者：
Mozdarani, Hossein;Nasirian, Borzo;Haeri, S. Abolghasem
通讯作者：
Haeri, S. Abolghasem
影响因子：
1
作者：
Roessler, U.;Hornhardt, S.;Gomolka, M.
通讯作者：
Gomolka, M.
DOI：
10.1016/j.mrgentox.2006.01.002
发表时间：
2006-04-30
影响因子：
1.9
作者：
Verde, PE;Geracitano, LA;Monserrat, JM
通讯作者：
Monserrat, JM
影响因子：
3.4
作者：
BENDER, MA;GOOCH, PC
通讯作者：
GOOCH, PC