Pinewood nematode-associated bacteria contribute to oxidative stress resistance of Bursaphelenchus xylophilus.
Pinewood nematode-associated bacteria contribute to oxidative stress resistance of Bursaphelenchus xylophilus.
复制标题
松木线虫相关的细菌有助于木囊杆菌的氧化应激抗性。
DOI：
10.1186/1471-2180-13-299
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发表时间：
2013-12-23
期刊：
影响因子：
4.2
通讯作者：
Hasegawa K
中科院分区：
文献类型：
--
作者：
Vicente CS;Ikuyo Y;Mota M;Hasegawa K
松材线虫（Bursaphelenchus xylophilus）引起的松材萎蔫病是世界上最严重的森林病害之一。最近，人们对嗜木杆菌相关细菌在PWD中的作用及其与线虫的相互作用进行了大量研究。一些研究报告了这种细菌对PWD发展的潜在贡献，要么是作为增强线虫致病性的助手，要么是作为表达与生活方式宿主适应相关的有趣性状的病原体。我们使用促氧化剂过氧化氢研究了严重氧化应激（OS）条件下线虫与细菌的相互作用，并探讨了这些细菌对线虫角质层表面的粘附能力。我们的研究结果清楚地表明，在OS条件下，沙雷氏菌（分离株LCN-4、LCN-16和pcn -146）对嗜木杆菌有有益的作用。研究发现，沙雷氏菌具有极强的os抗性，能促进嗜木芽孢杆菌的存活，并下调嗜木芽孢杆菌过氧化氢酶基因（Bxy-ctl-1和Bxy-ctl-2）。此外，我们发现在OS条件下，嗜木芽孢杆菌的毒力分离株（Ka4）比无毒分离株（C14-5）存活得更好。两种菌株的细菌效应都是横向的。我们没有观察到这些细菌在嗜木杆菌角质层表面有很强的特异性粘附。我们首次报道，嗜木芽胞杆菌相关细菌可能会在疾病中帮助线虫，并且有毒的嗜木芽胞杆菌分离物对OS条件的耐受性比无毒分离物更高。
Pine wilt disease (PWD) caused by the pinewood nematode Bursaphelenchus xylophilus is one of the most serious forest diseases in the world. The role of B. xylophilus-associated bacteria in PWD and their interaction with the nematode, have recently been under substantial investigation. Several studies report a potential contribution of the bacteria for the PWD development, either as a helper to enhance the pathogenicity of the nematode or as a pathogenic agent expressing interesting traits related to lifestyle host-adaptation. We investigated the nematode-bacteria interaction under a severe oxidative stress (OS) condition using a pro-oxidant hydrogen peroxide and explored the adhesion ability of these bacteria to the cuticle surface of the nematodes. Our results clearly demonstrated a beneficial effect of the Serratia spp. (isolates LCN-4, LCN-16 and PWN-146) to B. xylophilus under the OS condition. Serratia spp. was found to be extremely OS-resistant, and promote survival of B. xylophilus and down-regulate two B. xylophilus catalase genes (Bxy-ctl-1 and Bxy-ctl-2). In addition, we show that the virulent isolate (Ka4) of B. xylophilus survives better than the avirulent (C14-5) isolate under the OS condition. The bacterial effect was transverse for both B. xylophilus isolates. We could not observe a strong and specific adhesion of these bacteria on the B. xylophilus cuticle surface. We report, for the first time, that B. xylophilus associated bacteria may assist the nematode opportunistically in the disease, and that a virulent B. xylophilus isolate displayed a higher tolerance towards the OS conditions than an avirulent isolate.
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影响因子：
6.7
作者：
Kikuchi T;Cotton JA;Dalzell JJ;Hasegawa K;Kanzaki N;McVeigh P;Takanashi T;Tsai IJ;Assefa SA;Cock PJ;Otto TD;Hunt M;Reid AJ;Sanchez-Flores A;Tsuchihara K;Yokoi T;Larsson MC;Miwa J;Maule AG;Sahashi N;Jones JT;Berriman M
通讯作者：
Berriman M
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1.5
作者：
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通讯作者：
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影响因子：
7.4
作者：
MEHDY, MC
通讯作者：
MEHDY, MC
影响因子：
2.9
作者：
Ishiga, Yasuhiro;Uppalapati, Srinivasa Rao;Bender, Carol L.
通讯作者：
Bender, Carol L.
影响因子：
1.6
作者：
Kawazu, K;Zhang, H;Kanzaki, H
通讯作者：
Kanzaki, H