Killing of Treponema denticola by mouse peritoneal macrophages.
Killing of Treponema denticola by mouse peritoneal macrophages.
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DOI：
10.1177/0022034510363105
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发表时间：
2010-05
影响因子：
7.6
通讯作者：
Sambri V
中科院分区：
文献类型：
--
作者：
Gaibani P;Vocale C;Ambretti S;Cavrini F;Izard J;Miragliotta L;Pellegrino MT;Sambri V
齿垢密螺旋体已被确定为牙周病的一个重要原因，并假设参与口腔外感染。本研究的目的是探讨T.小鼠腹腔巨噬细胞体外摄取期间的齿垢细胞长度和运动性。在需氧和厌氧条件下培养的巨噬细胞在大肠杆菌LPS刺激下产生相似量的TNF-α。FlgE和CfpA缺陷突变体对T.由于细胞大小或缺乏运动性，与野生型菌株相比，齿垢显著增加。用特异性抗体调理显著提高了密螺旋体的摄取。这些结果表明，除了其他吞噬细胞外，巨噬细胞在T.特异性抗体的产生可提高对齿垢锥虫的免疫效果。在口腔部位或在传播过程中出现齿垢。
Treponema denticola has been identified as an important cause of periodontal disease and hypothesized to be involved in extra-oral infections. The objective of this study was to investigate the role of T. denticola cell length and motility during mouse peritoneal macrophages in vitro uptake. Macrophages, incubated under aerobic and anaerobic conditions, produced a similar amount of TNF-α when stimulated with Escherichia coli LPS. The uptake of FlgE- and CfpA-deficient mutants of T. denticola was significantly increased compared to the wild type strain due to cell size or lack of motility. Opsonization with specific antibodies considerably improved the treponemes’ uptake. These results suggest that macrophages, in addition to other phagocytes, could play an important role in the control of T. denticola infection and that the raising of specific antibodies could improve the efficacy of the immune response toward T. denticola either at an oral site or while disseminating.
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影响因子：
3.4
作者：
Magalhaes, Marco A. O.;Sun, Chun Xiang;Ellen, Richard P.
通讯作者：
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影响因子：
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作者：
Li, H;Ruby, J;Kuramitsu, H
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3.2
作者：
Limberger, RJ;Slivienski, LL;Samsonoff, WA
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Samsonoff, WA
影响因子：
3.2
作者：
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通讯作者：
Limberger, RJ
影响因子：
7.6
作者：
Foschi, F.;Izard, J.;Stashenko, P.
通讯作者：
Stashenko, P.