Comparison of suprachoroidal drug delivery with subconjunctival and intravitreal routes using noninvasive fluorophotometry.
Comparison of suprachoroidal drug delivery with subconjunctival and intravitreal routes using noninvasive fluorophotometry.
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DOI：
10.1371/journal.pone.0048188
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kompella UB
中科院分区：
文献类型：
--
作者：
Tyagi P;Kadam RS;Kompella UB
确定与玻璃体内和经巩膜途径相比，脉络膜上腔注射荧光素钠（NaF）对眼后段脉络膜-视网膜区域的暴露是否更大。脉络膜上注射，一种新的方法，用于药物输送到眼睛的后段进行了验证，使用34 G针和印度墨水注射在Sprague道利大鼠，然后进行组织学。在脉络膜上、后结膜下或玻璃体内注射后，在Sprague道利大鼠中比较NaF的递送。使用Fluorotron Master™（一种眼部荧光光度计）无创监测NaF水平长达6小时，使用WinNonlin估计药代动力学参数。组织学分析表明，注射后，印度墨水定位于巩膜下方的脉络膜上腔。NaF向脉络膜-视网膜的输送顺序为：脉络膜上腔>玻璃体内>后结膜下注射。与后结膜下注射（76±6 ng/ml）和玻璃体内注射（108±39 ng/ml）相比，脉络膜上注射（2744±1111 ng/ml）后脉络膜-视网膜中的NaF峰值浓度（Cmax）分别高36倍（p =0.001）和25倍（p=0.001）。 脉络膜上注射后脉络膜-视网膜的NaF暴露量（AUC 0 - 360 min）分别比后结膜下和玻璃体内注射高6倍（p= 0.001）和2倍（p =0.03）。 脉络膜上注射给药后立即观察到脉络膜-视网膜Tmax，结膜下和玻璃体内注射分别在10和27.5分钟观察到脉络膜-视网膜Tmax。脉络膜上注射在大鼠模型中是可行的。脉络膜上注射导致最高的生物利用度，也就是说，程度和速度的交付氟化钠脉络膜视网膜相比，玻璃体内和后结膜下注射。眼荧光光度法是有用的非侵入性监测NaF在大鼠给药后，通过各种途径，包括脉络膜上途径。
To determine whether exposure of sodium fluorescein (NaF) to the choroid-retina region in the posterior segment of the eye is greater with suprachoroidal injection when compared to intravitreal and transscleral routes. Suprachoroidal injection, a new approach for drug delivery to the posterior segment of the eye was validated using a 34 G needle and Indian ink injections in Sprague Dawley rats, followed by histology. Delivery of NaF was compared in Sprague Dawley rats after suprachoroidal, posterior subconjunctival, or intravitreal injections. NaF levels were monitored noninvasively up to 6 hours using Fluorotron Master™, an ocular fluorophotometer Pharmacokinetic parameters were estimated using WinNonlin. Histological analysis indicated localization of India ink to the suprachoroidal space below sclera, following injection. NaF delivery to choroid-retina was in the order: suprachoroidal > intravitreal >posterior subconjunctival injection. Peak NaF concentration (Cmax) in choroid-retina was 36-fold (p = 0.001) and 25-fold (p = 0.001) higher after suprachoroidal (2744±1111 ng/ml) injection when compared to posterior subconjunctival (76±6 ng/ml) and intravitreal (108±39 ng/ml) injections, respectively. NaF exposure (AUC0–360min) to choroid-retina after suprachoroidal injection was 6-fold (p = 0.001) and 2-fold (p = 0.03) higher than posterior subconjunctival and intravitreal injections, respectively. Choroid-retina Tmax was observed immediately after dosing with suprachoroidal injections and at 10 and 27.5 minutes, respectively, with subconjunctival and intravitreal injections. Suprachoroidal injections are feasible in a rat model. Suprachoroidal injections resulted in the highest bioavailability, that is, the extent and rate of delivery of NaF to choroid-retina, when compared to intravitreal and posterior subconjunctival injections. Ocular fluorophotometry is useful for noninvasive monitoring of NaF in rats following administration by various routes including suprachoroidal route.
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影响因子：
25.7
作者：
H端bner, GH;Steudel, N;Fleig, WE
通讯作者：
Fleig, WE
影响因子：
5.3
作者：
KATZ, ML;ROBISON, WG;BIERI, JG
通讯作者：
BIERI, JG
影响因子：
56.9
作者：
ELDRED, GE;MILLER, GV;FEENEYBURNS, L
通讯作者：
FEENEYBURNS, L
影响因子：
4.2
作者：
Olsen, Timothy W.;Feng, Xiao;Cameron, J. Douglas
通讯作者：
Cameron, J. Douglas
影响因子：
3.4
作者：
BATEY, DW;DANESHGAR, KK;ECKHERT, CD
通讯作者：
ECKHERT, CD