Actin-dependent propulsion of endosomes and lysosomes by recruitment of N-WASP.
Actin-dependent propulsion of endosomes and lysosomes by recruitment of N-WASP.
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DOI：
10.1083/jcb.148.3.519
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发表时间：
2000-02-07
期刊：
影响因子：
--
通讯作者：
Larabell CA
中科院分区：
文献类型：
--
作者：
Taunton J;Rowning BA;Coughlin ML;Wu M;Moon RT;Mitchison TJ;Larabell CA
我们研究了活爪蟾卵中肌动蛋白组装的空间和时间控制。卵子激活后几分钟内，富含肌动蛋白的动态彗尾出现在富含蛋白激酶 C (PKC) 的细胞质囊泡子集上，导致囊泡穿过细胞质。体内肌动蛋白彗尾的形成是由 PKC 激活剂佛波醇肉豆蔻酸酯乙酸酯 (PMA) 刺激的，并且该过程可以在无细胞系统中重建。我们使用该系统来定义区分与肌动蛋白彗星尾相关的囊泡与提取物中其他囊泡的特征。我们发现，N-WASP 蛋白被招募到与肌动蛋白彗星尾相关的每个囊泡的表面，这表明囊泡运动是由 Arp2/3 复合物成核的肌动蛋白组装引起的，Arp2/3 复合物是 N-WASP 的直接下游靶标。运动囊泡积累了吖啶橙染料，这是内体和溶酶体的标记物。此外，电子显微镜显示，与肌动蛋白彗星尾相关的囊泡具有多囊泡内体的形态特征。来自哺乳动物细胞的内体和溶酶体优先使肌动蛋白组装成核，并在非洲爪蟾卵提取物系统中移动。这些结果将内体和溶酶体定义为肌动蛋白成核机制的招募位点，并证明肌动蛋白组装有助于细胞器运动。相反，通过使肌动蛋白组装成核，细胞内膜可能有助于肌动蛋白细胞骨架的动态组织。
We examined the spatial and temporal control of actin assembly in living Xenopus eggs. Within minutes of egg activation, dynamic actin-rich comet tails appeared on a subset of cytoplasmic vesicles that were enriched in protein kinase C (PKC), causing the vesicles to move through the cytoplasm. Actin comet tail formation in vivo was stimulated by the PKC activator phorbol myristate acetate (PMA), and this process could be reconstituted in a cell-free system. We used this system to define the characteristics that distinguish vesicles associated with actin comet tails from other vesicles in the extract. We found that the protein, N-WASP, was recruited to the surface of every vesicle associated with an actin comet tail, suggesting that vesicle movement results from actin assembly nucleated by the Arp2/3 complex, the immediate downstream target of N-WASP. The motile vesicles accumulated the dye acridine orange, a marker for endosomes and lysosomes. Furthermore, vesicles associated with actin comet tails had the morphological features of multivesicular endosomes as revealed by electron microscopy. Endosomes and lysosomes from mammalian cells preferentially nucleated actin assembly and moved in the Xenopus egg extract system. These results define endosomes and lysosomes as recruitment sites for the actin nucleation machinery and demonstrate that actin assembly contributes to organelle movement. Conversely, by nucleating actin assembly, intracellular membranes may contribute to the dynamic organization of the actin cytoskeleton.
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基于肌动蛋白单核细胞增生李斯特菌的运动：肌动蛋白组装是由于细菌表面的固定细丝末端的局部维护而产生的。
影响因子：
7.8
作者：
MARCHAND, JB;MOREAU, P;PAOLETTI, A;COSSART, P;CARLIER, MF;PANTALONI, D
通讯作者：
PANTALONI, D
影响因子：
64.8
作者：
Loisel, TP;Boujemaa, R;Carlier, MF
通讯作者：
Carlier, MF
影响因子：
21.3
作者：
Merrifield, CJ;Moss, SE;Almers, W
通讯作者：
Almers, W
DOI：
10.1083/jcb.146.6.1319
发表时间：
1999-09-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Egile C;Loisel TP;Laurent V;Li R;Pantaloni D;Sansonetti PJ;Carlier MF
通讯作者：
Carlier MF
影响因子：
7.8
作者：
Bement, W M;Capco, D G
通讯作者：
Capco, D G