Deconstructing the Effects of Matrix Elasticity and Geometry in Mesenchymal Stem Cell Lineage Commitment.
Deconstructing the Effects of Matrix Elasticity and Geometry in Mesenchymal Stem Cell Lineage Commitment.
复制标题
DOI：
10.1002/adfm.201303400
复制
发表时间：
2014-04-23
影响因子：
19
通讯作者：
Jabbarzadeh, Ehsan
中科院分区：
文献类型：
--
作者：
Harris, Greg M.;Piroli, Maria E.;Jabbarzadeh, Ehsan
包括物理和生化信号在内的多种环境因素对干细胞的行为和功能负责。特别是，基质弹性和细胞形状已被证明决定干细胞的功能，但这些物理线索如何控制细胞分化之间的相互作用知之甚少。我们首次利用紫外线（UV）光刻技术对聚乙二醇（PEG）水凝胶进行成像，从而能够制造出能够分析基质弹性、细胞形状和细胞大小影响的微环境，从而探索间充质干细胞（MSC）谱系承诺中基质弹性和细胞形状之间的关系。我们的数据显示，无论基质弹性如何，在1000 μm2圆形、正方形和矩形上培养的细胞主要是脂肪形成的细胞系，而在2500 μm2和5000 μm2形状上培养的细胞更依赖于形状和弹性来确定细胞系。我们进一步描述了通过药物抑制剂修饰细胞骨架如何改变细胞行为。通过显示物理信号导致的间充质干细胞谱系承诺关系，本研究强调了细胞形状和基质弹性在进一步理解干细胞行为和未来组织工程策略中的重要性。
A wide variety of environmental factors including physical and biochemical signals are responsible for stem cell behavior and function. In particular, matrix elasticity and cell shape have been shown to determine stem cell function, yet little is known about the interplay between how these physical cues control cell differentiation. For the first time, by using ultraviolet (UV) lithography to pattern poly(ethylene) glycol (PEG) hydrogels we are able to manufacture microenvironments capable of parsing the effects of matrix elasticity, cell shape, and cell size in order to explore the relationship between matrix elasticity and cell shape in mesenchymal stem cell (MSC) lineage commitment. Our data shows that cells cultured on 1,000 μm2 circles, squares, and rectangles were primarily adipogenic lineage regardless of matrix elasticity, while cells cultured on 2,500 and 5,000 μm2 shapes more heavily depended on shape and elasticity for lineage specification. We further went on to characterize how modifying the cell cytoskeleton through pharmacological inhibitors can modify cell behavior. By showing MSC lineage commitment relationships due to physical signals, this study highlights the importance of cell shape and matrix elasticity in further understanding stem cell behavior for future tissue engineering strategies.
登录
查看更多内容
影响因子：
5.3
作者：
Docheva D;Padula D;Popov C;Mutschler W;Clausen-Schaumann H;Schieker M
通讯作者：
Schieker M
DOI：
10.1073/pnas.0903269107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Kilian, Kristopher A.;Bugarija, Branimir;Mrksich, Milan
通讯作者：
Mrksich, Milan
影响因子：
3.1
作者：
Crisostomo, Paul R.;Wang, Meijing;Meldrum, Daniel R.
通讯作者：
Meldrum, Daniel R.
影响因子：
3.9
作者：
Gunawan, RC;Silvestre, J;Leckband, DE
通讯作者：
Leckband, DE
DOI：
10.1083/jcb.200405004
发表时间：
2004-09-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Engler AJ;Griffin MA;Sen S;Bönnemann CG;Sweeney HL;Discher DE
通讯作者：
Discher DE