Researching into the cellular shape, volume and elasticity of mesenchymal stem cells, osteoblasts and osteosarcoma cells by atomic force microscopy.
Researching into the cellular shape, volume and elasticity of mesenchymal stem cells, osteoblasts and osteosarcoma cells by atomic force microscopy.
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DOI：
10.1111/j.1582-4934.2007.00138.x
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发表时间：
2008-04
影响因子：
5.3
通讯作者：
Schieker M
中科院分区：
文献类型：
--
作者：
Docheva D;Padula D;Popov C;Mutschler W;Clausen-Schaumann H;Schieker M
在骨骼中有几种不同类型的细胞，包括成骨细胞(OBS)和间充质干细胞(MSCs)。骨髓间充质干细胞具有向成骨细胞分化的潜能，是骨再生医学的理想靶点。人骨髓间充质干细胞具有两种不同的形态：快速自我更新细胞(RS)和增殖率极低的扁平细胞(FC)。在病理性骨骼疾病中发现的另一种细胞类型是骨肉瘤。在这项研究中，我们用原子力显微镜(AFM)比较了RS和FC细胞，人OBS和MG63骨肉瘤细胞的形貌和形态计量学特征。结果显示出明显的差异：FC和HOB细胞具有相似的褶皱形态，而RS和MG63细胞具有更光滑的表面。此外，我们还研究了选定的底物对细胞形态计量学的影响。我们发现，RS和MG63细胞在聚苯乙烯和I型胶原等纤维基质上更平坦，但在玻璃上更圆，玻璃是最光滑的表面。相反，面积较大的细胞，即FC和HOB细胞，对于不同的底物，平坦度没有明显的变化。然而，与RS和MG63细胞相比，它们明显更平坦。我们可以用粘着的程度来解释平整度的不同。事实上，FC和HOB细胞显示出更高的局灶性粘连含量。最后，我们用原子力显微镜测定了细胞的杨氏模数。RS、FC和HOB细胞在三种不同的基质上表现出相似的硬度，而MG63细胞表现出在I型胶原上弹性增加的独特特征。综上所述，我们的结果首次显示了来自正常和病理骨的不同类型的人类细胞的形态计量学和生物物理特征之间的直接比较。我们的研究表明，除了RNA、蛋白质组和功能研究外，细胞的形态和生物力学特征也揭示了新的细胞特征和相互关系。
Within the bone lie several different cell types, including osteoblasts (OBs) and mesenchymal stem cells (MSCs). The MSCs are ideal targets for regenerative medicine of bone due to their differentiation potential towards OBs. Human MSCs exhibit two distinct morphologies: rapidly self-renewing cells (RS) and flat cells (FC) with very low proliferation rates. Another cell type found in pathological bone conditions is osteosarcoma. In this study, we compared the topographic and morphometric features of RS and FC cells, human OBs and MG63 osteosarcoma cells by atomic force microscopy (AFM). The results demonstrated clear differences: FC and hOB cells showed similar ruffled topography, whereas RS and MG63 cells exhibited smoother surfaces. Furthermore, we investigated how selected substrates influence cell morphometry. We found that RS and MG63 cells were flatter on fibrous substrates such as polystyrene and collagen I, but much more rounded on glass, the smoothest surface. In contrast, cells with large area, namely FC and hOB cells, did not exhibit pronounced changes in flatness with regards to the different substrates. They were, however, remarkably flatter in comparison to RS and MG63 cells. We could explain the differences in flatness by the extent of adhesion. Indeed, FC and hOB cells showed much higher content of focal adhesions. Finally, we used the AFM to determine the cellular Young's modulus. RS, FC and hOB cells showed comparable stiffness on the three different substrates, while MG63 cells demonstrated the unique feature of increased elasticity on collagen I. In summary, our results show, for the first time, a direct comparison between the morphometric and biophysical features of different human cell types derived from normal and pathological bone. Our study manifests the opinion that along with RNA, proteomic and functional research, morphological and biomechanical characterization of cells also reveals novel cell features and interrelationships.
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影响因子：
5.8
作者：
Domke, J;Dannöhl, S;Radmacher, M
通讯作者：
Radmacher, M
影响因子：
4.2
作者：
Huang, George T-J.;Shagramanova, Kristina;Chan, Selina W.
通讯作者：
Chan, Selina W.
影响因子：
--
作者：
Costa KD
通讯作者：
Costa KD
影响因子：
7
作者：
Darling, E. M.;Zauscher, S.;Guilak, F.
通讯作者：
Guilak, F.
影响因子：
3.5
作者：
Boecker, Wolfgang;Rossmann, Oliver;Schieker, Matthias
通讯作者：
Schieker, Matthias