In vivo studies investigating biodistribution of nanoparticle-encapsulated rhodamine B delivered via dissolving microneedles.
In vivo studies investigating biodistribution of nanoparticle-encapsulated rhodamine B delivered via dissolving microneedles.
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DOI：
10.1016/j.jconrel.2017.04.022
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发表时间：
2017-11-10
期刊：
影响因子：
--
通讯作者：
Donnelly RF
中科院分区：
文献类型：
--
作者：
Kennedy J;Larrañeta E;McCrudden MTC;McCrudden CM;Brady AJ;Fallows SJ;McCarthy HO;Kissenpfennig A;Donnelly RF
纳米颗粒（NP）作为药物输送和靶向载体已经经过了广泛的研究。 NP 递送通常通过肠胃外途径进行，依赖于使用皮下注射针进行给药，这可能与患者依从性问题和安全问题相关。最近，通过新型可溶性微针（MN）阵列进行纳米颗粒的皮内递送引起了制药界的兴趣。然而，使用这种组合方法的已发表研究有限，因为它们仅关注体外和离体模型的使用。目前的研究旨在回答这样的基本问题：在 MN 介导的递送后，此类 NP 如何在体内小鼠模型中分布。罗丹明 B (RhB) 被用作模型示踪染料，以促进生物分布的研究。应用 MN 后，在小鼠的肝脏、肾脏、脾脏和腮腺浅淋巴结中检测到了 RhB。淋巴管的吸收尤其值得注意，因为它表明了利用微创 MN 递送策略将活性药物和疫苗控制靶向淋巴管的潜力。经过进一步开发，这种递送系统的使用可以在增强免疫调节和抗癌治疗方面产生深远的益处。因此，有必要进一步研究 MN/NP 组合递送策略。
Nanoparticles (NPs) have undergone extensive investigation as drug delivery and targeting vehicles. NP delivery is often via the parenteral route, reliant on administration using hypodermic needles, which can be associated with patient compliance issues and safety concerns. In the recent past, the intradermal delivery of NPs, via novel dissolving microneedle (MN) arrays has garnered interest in the pharmaceutical community. However, published studies using this combinatorial approach have been limited, in that they have focussed on the use of in vitro and ex vivo models only. The current study was designed to answer the fundamental question of how such NPs are distributed in an in vivo murine model, following MN-mediated delivery. Rhodamine B (RhB) was employed as a model tracer dye to facilitate study of biodistribution. Following MN application, RhB was detected in the livers, kidneys, spleens and superficial parotid lymph nodes of the mice. Uptake into the lymphatics was of particular note, as it points towards the potential for utilisation of a minimally-invasive MN delivery strategy in controlled targeting of active drug substances and vaccines to the lymphatics. The use of such a delivery system could, following further development, have far-reaching benefits in enhancement of immunomodulatory and anti-cancer therapies. As a consequence, further investigation of MN/NP combinatorial delivery strategies is warranted.
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影响因子：
3.8
作者：
Garcia-Perez, Luis-Emilio;Alvarez, Maria;Dilla, Tatiana;Gil-Guillen, Vicente;Orozco-Beltran, Domingo
通讯作者：
Orozco-Beltran, Domingo
影响因子：
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作者：
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通讯作者：
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影响因子：
2.4
作者：
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通讯作者：
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