Expression of the vascular endothelial growth factor receptor, KDR, in human placenta.
Expression of the vascular endothelial growth factor receptor, KDR, in human placenta.
复制标题
血管内皮生长因子受体 KDR 在人胎盘中的表达。
作者：
M. Vučković;J. Ponting;B. I. Terman;V. Niketić;M. Seif;Shant Kumar
血管内皮生长因子(VEGF)是一种肝素结合性生长因子，通过促进细胞增殖和通透性直接作用于血管内皮细胞。到目前为止，已经鉴定出3种与血管内皮生长因子结构相关的细胞表面受体，即Flt-1、Flt-4和KDR，它们都是人Ⅲ型受体酪氨酸激酶。血管网络的建立对胎盘的发育至关重要，它通过血管生成和血管生成两种途径发生。控制这些过程的信号尚不清楚。免疫组织化学和原位杂交技术已将血管内皮生长因子定位于滋养层，并与胎盘血管内皮细胞和血管母细胞结合，提示血管内皮生长因子及其受体在血管网络的发育中起作用。在这项研究中，我们使用了特定的抗体来定位KDR和血管内皮细胞在妊娠1和3个月的人胎盘。染色显示KDR与内皮细胞和血管母细胞共存。未观察到滋养细胞的染色，但在滋养细胞增生区附近的绒毛基质中可见内皮细胞的强烈染色。
Vascular endothelial growth factor (VEGF) is a heparin-binding growth factor known to act directly on vascular endothelial cells by promoting cell proliferation and permeability. To date, 3 structurally related cell surface receptors for VEGF, Flt-1, Flt-4 and KDR, have been identified and shown to be human type III receptor tyrosine kinases. The establishment of a vascular network is crucial to the development of the placenta and occurs through both angiogenesis and vasculogenesis. The signals controlling these processes are unclear. Immunohistochemical and in situ hybridisation techniques have localised VEGF in the trophoblast layers and VEGF binding to placental vascular endothelial cells and haemangioblasts has been shown, suggesting a role for VEGF and its receptors in development of the vascular network. In this study we have used specific antibodies to localise KDR and endothelial cells in 1st and 3rd trimester human placenta. The staining showed a colocalisation of KDR with endothelial cells and haemangioblasts. No staining of trophoblast cells was observed, but strong staining of the endothelial cells was seen in the villous stroma adjacent to areas of trophoblast proliferation.
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影响因子：
4.4
作者：
A. Koch;A. Koch;L. Harlow;G. K. Haines;E. Amento;E. Unemori;Wei Lee Wong;R. Pope;N. Ferrara
通讯作者：
A. Koch;A. Koch;L. Harlow;G. K. Haines;E. Amento;E. Unemori;Wei Lee Wong;R. Pope;N. Ferrara
影响因子：
56.9
作者：
DEVRIES, C;ESCOBEDO, JA;WILLIAMS, LT
通讯作者：
WILLIAMS, LT
血管内皮生长因子信使核糖核酸在灵长类动物卵巢中的表达。
影响因子：
4.8
作者：
Ravindranath,N;Little-Ihrig,L;Phillips,HS;Ferrara,N;Zeleznik,AJ
通讯作者：
Zeleznik,AJ
DOI：
10.1016/0955-2235(89)90012-4
发表时间：
1989-01-01
期刊：
Progress in growth factor research
影响因子：
--
作者：
Klagsbrun, M
通讯作者：
Klagsbrun, M