Mutational pathways and genetic barriers to CXCR4-mediated entry by human immunodeficiency virus type 1.
Mutational pathways and genetic barriers to CXCR4-mediated entry by human immunodeficiency virus type 1.
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DOI：
10.1016/j.virol.2010.09.026
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发表时间：
2011-01-20
期刊：
影响因子：
3.7
通讯作者：
Petropoulos CJ
中科院分区：
文献类型：
--
作者：
Huang W;Frantzell A;Toma J;Fransen S;Whitcomb JM;Stawiski E;Petropoulos CJ
To examine mutational pathways that lead to CXCR4 use of HIV-1, we analyzed the genotypic and phenotypic characteristics of envelope sequences from a large panel of patient virus populations and individual clones containing different V3 mutations. Basic amino acid substitutions at position 11 were strong determinants of CXCR4-mediated entry, but required multiple compensatory mutations to overcome associated reductions in infectivity. In contrast, basic amino acid substitutions at position 25, or substitutions at position 6–8 resulting in the loss of a potential N-linked glycosylation site, contributed to CXCR4-mediated entry, but required additional substitutions acting cooperatively to confer efficient CXCR4 use. Our assumptions, based upon examination of patient viruses, were largely confirmed by characterizing the coreceptor utilization of five distinct panels of isogenic envelope sequences containing V3 amino acid substitutions introduced by site-directed mutagenesis. These results further define the mutational pathways leading to CXCR4 use and their associated genetic barriers.
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影响因子：
56.9
作者：
HWANG, SS;BOYLE, TJ;CULLEN, BR
通讯作者：
CULLEN, BR
影响因子：
64.8
作者：
Deng, HK;Liu, R;Landau, NR
通讯作者：
Landau, NR
DOI：
10.1056/nejmoa0803152
发表时间：
2008-10-02
期刊：
The New England journal of medicine
影响因子：
--
作者：
Gulick RM;Lalezari J;Goodrich J;Clumeck N;DeJesus E;Horban A;Nadler J;Clotet B;Karlsson A;Wohlfeiler M;Montana JB;McHale M;Sullivan J;Ridgway C;Felstead S;Dunne MW;van der Ryst E;Mayer H;MOTIVATE Study Teams
通讯作者：
MOTIVATE Study Teams
影响因子：
--
作者：
Hoffman, TL;Doms, RW
通讯作者：
Doms, RW
影响因子：
1.5
作者：
Cardozo, Timothy;Kimura, Tetsuya;Zolla-Pazner, Susan
通讯作者：
Zolla-Pazner, Susan