A highly differentiated region of wheat chromosome 7AL encodes a Pm1a immune receptor that recognizes its corresponding AvrPm1a effector from Blumeria graminis.
A highly differentiated region of wheat chromosome 7AL encodes a Pm1a immune receptor that recognizes its corresponding AvrPm1a effector from Blumeria graminis.
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小麦染色体7AL的一个高度分化区域编码Pm1a免疫受体，该受体可识别来自蓝毛菌的相应AvrPm1a效应体。
DOI：
10.1111/nph.17075
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Zhang P
中科院分区：
文献类型：
--
作者：
Hewitt T;Müller MC;Molnár I;Mascher M;Holušová K;Šimková H;Kunz L;Zhang J;Li J;Bhatt D;Sharma R;Schudel S;Yu G;Steuernagel B;Periyannan S;Wulff B;Ayliffe M;McIntosh R;Keller B;Lagudah E;Zhang P
Pm1a是在小麦中发现的首个抗白粉病基因，是位于染色体7AL远端区域的复杂抗性(R)基因簇的一部分，该基因簇抑制遗传重组。利用诱变和R基因富集测序技术（MutRenSeq）分离了一个核苷酸结合、富含亮氨酸重复序列（NLR）的免疫受体基因。稳定转化证实了Pm1a的身份，该身份在转基因植物中诱导了对无毒小麦白粉病病原体的强抗性表型。结合病原体基因组重测序和RNA测序（RNAseq）技术，一种分离Pm1a毒性的禾草芽孢杆菌家族高密度遗传图谱确定了AvrPm1a效应基因候选株。在植物表达中，发现了一个具有N端Y/FxC基序的效应子，当与benthamiana Pm1a共表达时，会引起强烈的过敏反应。单染色体富集测序（ChromSeq）和染色体7A的组装表明，Pm1a区域周围的重组抑制是由于染色体7A、7B和7D的重排。Pm1a的克隆及其在7A染色体高度重排区域的鉴定为染色体重排在这一复杂抗性簇进化中的作用提供了深入的见解。
Pm1a, the first powdery mildew resistance gene described in wheat, is part of a complex resistance (R) gene cluster located in a distal region of chromosome 7AL that has suppressed genetic recombination. A nucleotide‐binding, leucine‐rich repeat (NLR) immune receptor gene was isolated using mutagenesis and R gene enrichment sequencing (MutRenSeq). Stable transformation confirmed Pm1a identity which induced a strong resistance phenotype in transgenic plants upon challenge with avirulent Blumeria graminis (wheat powdery mildew) pathogens. A high‐density genetic map of a B. graminis family segregating for Pm1a avirulence combined with pathogen genome resequencing and RNA sequencing (RNAseq) identified AvrPm1a effector gene candidates. In planta expression identified an effector, with an N terminal Y/FxC motif, that induced a strong hypersensitive response when co‐expressed with Pm1a in Nicotiana benthamiana. Single chromosome enrichment sequencing (ChromSeq) and assembly of chromosome 7A suggested that suppressed recombination around the Pm1a region was due to a rearrangement involving chromosomes 7A, 7B and 7D. The cloning of Pm1a and its identification in a highly rearranged region of chromosome 7A provides insight into the role of chromosomal rearrangements in the evolution of this complex resistance cluster.
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