Significant association of SNP polymorphism in the tilapia enhancer of polycomb homolog 1 gene with salt tolerance
Significant association of SNP polymorphism in the tilapia enhancer of polycomb homolog 1 gene with salt tolerance
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罗非鱼多梳同源物1基因增强子SNP多态性与耐盐性显着相关
DOI：
10.1111/are.13625
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发表时间：
2018-04
影响因子：
2
通讯作者：
Jun Hong Xia
中科院分区：
文献类型：
--
作者：
Xiao Hui Gu;Hao Ran Lin;Jun Hong Xia
筛选出与渗透压调节相关的候选基因，为开发盐渍罗非鱼育种分子标记奠定了基础。本研究对尼罗罗非鱼多梳同源基因增强子1(EPC1)进行了鉴定和功能分析。EPC1CDS序列全长1161个碱基，包括14个外显子，编码386个氨基酸残基。用qRT-PCR方法研究了盐度分别为0、10、15和20 ppt的尼罗罗非鱼的鳃、脑和肠道组织中EPC1基因的表达。我们发现，在20ppt的高盐度胁迫下，该基因显著下调。我们还通过对一个全同胞罗非鱼家系(N=1~500)的192个极端个体进行基因分型，发现了EPC1基因的5个SNP与耐盐性有关的显著证据。群体中SNP杂合子个体(平均生存时间3,064天，S)明显低于其他纯合子个体(平均生存时间5,986天，S)。对生活在不同盐度环境中的31种鱼类的EPC1蛋白序列进行了进一步的功能分析，发现7个氨基酸位点(α)与盐度含量显著相关。这些数据表明EPC1基因可能是罗非鱼渗透调节过程中的一个候选基因。我们的研究结果可能有助于利用分子标记辅助选择技术进行盐碱罗非鱼的选育。
Identifying candidate genes involved into osmoregulation provides a basis for developing molecular markers for breeding of saline tilapia. In this study, we characterized and conducted a functional analysis of theEnhancer of Polycomb Homolog 1(EPC1) gene in Nile tilapia. The length of theEPC1CDS sequence was 1161 bp, including 14 exons encoding 386 amino acid residues. The expression forEPC1was investigated in the gill, brain and intestine tissues of Nile tilapia that challenged by 0 ppt, 10 ppt, 15 ppt and 20 ppt of salinity content by qRT‐PCR. We found that the gene was significantly down‐regulated at 20 ppt of high salinity stress. We also detected significant evidence of 5 SNP association in theEPC1gene with salt tolerance trait by genotyping 192 extreme individuals from a full‐sib tilapia family (N= ~500). The individuals with heterozygous SNP genotypes in the population (with an average survival time of 3,064 s) were significantly less tolerant than the other individuals with the homozygote genotypes (with an average survival time of 5,986 s). Further functional analysis on theEPC1protein sequences from 31 fish species inhabiting different salinity environments identified seven amino acid sites as significantly associated sites (α < 0.01) with salinity content. These data suggested that theEPC1gene may be a candidate gene related to osmoregulation process in tilapia. Our findings could contribute to selection of the saline tilapia by using marker‐assisted selection technique.
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