Salamander limb regeneration involves the activation of a multipotent skeletal muscle satellite cell population.
Salamander limb regeneration involves the activation of a multipotent skeletal muscle satellite cell population.
复制标题
DOI：
10.1083/jcb.200509011
复制
发表时间：
2006-01-30
期刊：
影响因子：
--
通讯作者：
Simon A
中科院分区：
文献类型：
--
作者：
Morrison JI;Lööf S;He P;Simon A
与哺乳动物相比，蝾螈在受伤后可以再生复杂的结构，包括整个四肢。一个核心问题是，在肢体再生和哺乳动物组织修复过程中，祖细胞的产生是通过单独的还是重叠的机制发生的。肢体的再生依赖于胚芽的形成，新的附属物从胚芽发育而来。残肢组织（如骨骼肌）的去分化先于胚泡形成，但不知道去分化是否涉及干细胞活化。我们描述了位于蝾螈肢体骨骼肌内的多能Pax7+卫星细胞群。我们证明骨骼肌去分化涉及卫星细胞激活，这些细胞可以促进新的肢体组织。蝾螈卫星细胞的激活方式类似于哺乳动物肌纤维在骨骼肌组织修复过程中如何动员干细胞。因此，肢体再生和哺乳动物组织修复具有共同的细胞和分子程序。我们的研究结果还确定卫星细胞是促进哺乳动物胚泡形成的潜在靶点。
In contrast to mammals, salamanders can regenerate complex structures after injury, including entire limbs. A central question is whether the generation of progenitor cells during limb regeneration and mammalian tissue repair occur via separate or overlapping mechanisms. Limb regeneration depends on the formation of a blastema, from which the new appendage develops. Dedifferentiation of stump tissues, such as skeletal muscle, precedes blastema formation, but it was not known whether dedifferentiation involves stem cell activation. We describe a multipotent Pax7+ satellite cell population located within the skeletal muscle of the salamander limb. We demonstrate that skeletal muscle dedifferentiation involves satellite cell activation and that these cells can contribute to new limb tissues. Activation of salamander satellite cells occurs in an analogous manner to how the mammalian myofiber mobilizes stem cells during skeletal muscle tissue repair. Thus, limb regeneration and mammalian tissue repair share common cellular and molecular programs. Our findings also identify satellite cells as potential targets in promoting mammalian blastema formation.
登录
查看更多内容
影响因子：
7.8
作者：
Asakura, Atsushi;Seale, Patrick;Girgis-Gabardo, Adele;Rudnicki, Michael A
通讯作者：
Rudnicki, Michael A
影响因子：
64.8
作者：
CAMERON, JA;HILGERS, AR;HINTERBERGER, TJ
通讯作者：
HINTERBERGER, TJ
影响因子：
2.7
作者：
HAY, ED
通讯作者：
HAY, ED
影响因子：
4.6
作者：
Gargioli, C;Slack, JMW
通讯作者：
Slack, JMW
影响因子：
2.7
作者：
Cornelison, DDW;Wold, BJ
通讯作者：
Wold, BJ