Bone marrow stromal cells contribute to bone formation following infusion into femoral cavities of a mouse model of osteogenesis imperfecta.
Bone marrow stromal cells contribute to bone formation following infusion into femoral cavities of a mouse model of osteogenesis imperfecta.
复制标题
DOI：
10.1016/j.bone.2010.05.040
复制
发表时间：
2010-09
期刊：
影响因子：
4.1
通讯作者：
Niyibizi, Christopher
中科院分区：
文献类型：
--
作者：
Li, Feng;Wang, Xujun;Niyibizi, Christopher
目前，关于骨髓基质细胞（BMSCs）在受体动物体内系统递送时对骨形成的贡献，文献中存在相互矛盾的数据。为了了解骨髓间充质干细胞是否有助于成骨不全骨（OI）的骨细胞表型和骨形成，将标记有GFP的骨髓间充质干细胞直接注入成骨不全小鼠模型的股骨中（oim）。通过组织学、免疫组织化学和受体骨的生物力学载荷来评估细胞对细胞表型和骨形成的贡献。骨髓间充质干细胞输注两周后，与注入生理盐水的股骨相比，受体股骨的组织学检查显示存在新骨，而注入盐水的股骨很少或没有骨形成。GFP荧光显示，新骨含有少量供体细胞。在细胞注射后6周，受体股骨中仍可检测到新骨，但通过注射悬浮在胃蛋白酶溶解型胶原中的细胞增强了新骨。免疫荧光和免疫组织化学染色显示，供体GFP阳性细胞在新生骨中与骨钙素表达细胞定位，提示这些细胞在体内已分化为成骨细胞。生物力学加载到三点弯曲失效时，结果显示，与单独注射PBS或I型胶原相比，注入骨髓间充质干细胞的股骨在生物力学上更强。综上所述，移植细胞在体内分化为成骨细胞并促进体内骨形成；我们还推测，供体细胞诱导分化或募集内源性细胞在移植后的早期阶段启动修复过程。
Currently, there are conflicting data in literature regarding contribution of bone marrow stromal cells (BMSCs) to bone formation when the cells are systemically delivered in recipient animals. To understand if BMSCs contribute to bone cell phenotype and bone formation in osteogenesis imperfecta bones (OI), MSCs marked with GFP were directly infused into the femurs of a mouse model of OI (oim). The contribution of the cells to the cell phenotype and bone formation was assessed by histology, immunohistochemistry and biomechanical loading of recipient bones. Two weeks following infusion of BMSCs, histological examination of the recipient femurs demonstrated presence of new bone when compared to femurs injected with saline which showed little or no bone formation. The new bone contained few donor cells as demonstrated by GFP fluorescence. At six weeks following cell injection, new bone was still detectable in the recipient femurs but was enhanced by injection of the cells suspended in pepsin solublized type I collagen. Immunofluorescence and immunohistochemical staining showed that donor GFP positive cells in the new bone were localized with osteocalcin expressing cells suggesting that the cells differentiated into osteoblasts in vivo. Biomechanical loading to failure in thee point bending, revealed that, femurs infused with BMSCs in PBS or in soluble type I collagen were biomechanically stronger than those injected with PBS or type I collagen alone. Taken together, the results indicate that transplanted cells differentiated into osteoblasts in vivo and contributed to bone formation in vivo; we also speculate that donor cells induced differentiation or recruitment of endogenous cells to initiate reparative process at early stages following transplantation.
登录
查看更多内容
影响因子：
20.3
作者：
Panaroni, Cristina;Gioia, Roberta;Forlino, Antonella
通讯作者：
Forlino, Antonella
DOI：
10.1016/j.bbrc.2008.08.138
发表时间：
2008-11-07
影响因子：
3.1
作者：
Lee, Sang Yang;Nakagawa, Toshiyuki;Reddi, A. Hari
通讯作者：
Reddi, A. Hari
影响因子：
82.9
作者：
Bi, Yanming;Ehirchiou, Driss;Young, Marian F.
通讯作者：
Young, Marian F.
影响因子：
5.2
作者：
Gnecchi, Massimiliano;He, Huamei;Melo, Luis G.;Noiseaux, Nicolas;Morello, Fulvio;de Boer, Rudolf A.;Zhang, Lunan;Pratt, Richard E.;Dzau, Victor J.;Ingwall, Joanne S.
通讯作者：
Ingwall, Joanne S.
DOI：
10.2217/17584272.4.1.57
发表时间：
2009-02-01
期刊：
International journal of clinical rheumatology
影响因子：
--
作者：
Niyibizi C;Li F
通讯作者：
Li F