Skin Treatment with Detergent Induces Dermatitis with H1-Antihistamine-Refractory Itch and Upregulates IL-4 and Th17/Th22 Cytokine Gene Expression in C57BL/6 Mice.
Skin Treatment with Detergent Induces Dermatitis with H1-Antihistamine-Refractory Itch and Upregulates IL-4 and Th17/Th22 Cytokine Gene Expression in C57BL/6 Mice.
复制标题
DOI：
10.1159/000525656
复制
发表时间：
2022
影响因子：
2.8
通讯作者：
Ikeda, Shigaku
中科院分区：
文献类型：
--
作者：
Masutani, Yurie;Takai, Toshiro;Kamijo, Seiji;Kimitsu, Toru;Yoshimura, Tomoko;Ichikawa, Saori;Shimizu, Saya;Ogawa, Takasuke;Takada, Keiko;Tominaga, Mitsutoshi;Suto, Hajime;Takamori, Kenji;Ogawa, Hideoki;Okumura, Ko;Ikeda, Shigaku
关键词：
皮肤反复接触洗涤剂会导致慢性刺激性接触性皮炎(ICD)，并伴有瘙痒和湿疹。然而，洗涤剂诱导ICD的机制尚不清楚。在这里，我们建立了一种新的小鼠洗涤剂诱导的ICD模型，并伴有H1-抗组胺-顽固性瘙痒。对C57BL/6基因背景的野生型和肥大细胞缺陷小鼠的耳部皮肤进行了去污剂十二烷基硫酸钠(十二烷基硫酸钠)治疗，持续约2周，同时给予或不给予H1-抗组胺药非索非那丁。分析皮肤炎症、屏障功能障碍和瘙痒。用定量聚合酶链式反应检测耳垂基因表达，用流式细胞仪分析引流淋巴结细胞。在野生型和肥大细胞缺陷小鼠中，十二烷基硫酸钠处理引起皮肤炎症和耳肿胀，增加经皮失水和后爪抓挠行为。在最后一次十二烷基硫酸钠处理后，刮擦次数的峰值至少保持了48小时。H1-抗组胺药物治疗的反应没有减少或几乎没有减少。与未治疗组相比，脾虚组小鼠外周血中Th2型细胞因子IL-4和Th17/Th22细胞因子IL-17A、IL-17F和IL-22的基因表达上调，CD4T、CD8T和γδT细胞引流淋巴结中的细胞数增加，GATA3、RoRγt、T-bet或FOXP3表达增强。本研究表明，十二烷基硫酸钠对C57BL/6小鼠耳部皮肤的处理可诱导非肥大细胞非依赖性皮肤炎症，表现为H1-抗组胺难治性瘙痒，提示该模型可能存在Th细胞因子和/或淋巴细胞介导的调节。该模型将有助于阐明洗涤剂诱导的ICD中H1-抗组胺-顽固性瘙痒的炎症机制。
Repeated skin contact to detergents causes chronic irritant contact dermatitis (ICD) associated with itch sensation and eczema. However, the mechanisms of detergent-induced ICD are poorly understood. Here, we established a new murine model of detergent-induced ICD with H1-antihistamine-refractory itch. Ear skin of wild-type and mast cell-deficient mice on the C57BL/6 genetic background was treated with a detergent, sodium dodecyl/lauryl sulfate (SDS), daily for approximately 2 weeks with or without administration of an H1-antihistamine, fexofenadine. Skin inflammation, barrier dysfunction, and itching were analyzed. Quantitative PCR for earlobe gene expression and flow cytometry analysis for draining lymph node cells were conducted. SDS treatment induced skin inflammation with ear swelling, increased transepidermal water loss, and hind-paw scratching behaviors in the wild-type and mast cell-deficient mice. The peak value of scratching bouts was retained for at least 48 h after the last SDS treatment. H1-antihistamine administration showed no or little reduction in the responses. SDS treatment upregulated gene expression for a Th2 cytokine IL-4 and Th17/Th22 cytokines, IL-17A, IL-17F, and IL-22, and increased cell numbers in draining lymph nodes of CD4<sup>+</sup> T, CD8<sup>+</sup> T, and γδT cells with enhanced expression of GATA3, RORγt, T-bet, or FOXP3 compared with untreated mice. The present study showed that SDS treatment of ear skin in C57BL/6 mice induces mast cell-independent skin inflammation with H1-antihistamine-refractory itch and suggested a possible Th cytokine- and/or lymphocyte-mediated regulation of the model. The model would be useful for elucidation of mechanisms for inflammation with H1-antihistamine-refractory itch in detergent-induced ICD.
登录
查看更多内容
影响因子：
4
作者：
Hristodorov, Dmitrij;Mladenov, Radoslav;Thepen, Theo
通讯作者：
Thepen, Theo
影响因子：
14.2
作者：
Simons, F. Estelle R.;Simons, Keith J.
通讯作者：
Simons, Keith J.
影响因子：
3.6
作者：
Inami, Yoshihiro;Sasaki, Atsushi;Kuraishi, Yasushi
通讯作者：
Kuraishi, Yasushi
DOI：
10.1016/j.jaci.2017.01.011
发表时间：
2017-04
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Brunner PM;Guttman-Yassky E;Leung DY
通讯作者：
Leung DY
影响因子：
3.6
作者：
de Jongh, Cindy M.;Lutter, Rene;Kezic, Sanja
通讯作者：
Kezic, Sanja