Auxin Induction of Cell Cycle Regulated Activity of Tobacco Telomerase*
Auxin Induction of Cell Cycle Regulated Activity of Tobacco Telomerase*
复制标题
生长素诱导烟草端粒酶的细胞周期调节活性*
DOI：
--
复制
发表时间：
1999
影响因子：
4.8
通讯作者：
Hideo Takahashi
中科院分区：
文献类型：
--
作者：
K. Tamura;Hongtu Liu;Hideo Takahashi
用端粒重复序列扩增法检测了悬浮培养的同步化烟草(Nictiana Tabacum)BY-2细胞在细胞周期各时相的端粒酶活性。在细胞周期的大多数时相，该酶的活性都很低或检测不到，但在S期早期，活性显着增加。端粒酶活性的诱导不受S期阻断剂阿昔双林(抑制α聚合酶)和羟基脲(抑制核苷酸还原酶)的影响，但可被阻断G1-S细胞周期转换的CDC2/CDK2激酶的抑制剂olomouine所阻止。这些结果表明，端粒酶活性的诱导并不直接与传统的DNA聚合酶的复制偶联，而是由细胞进入S期触发的。植物激素生长素的不同类似物，包括吲哚-3-乙酸、α-萘乙酸和2，4-二氯苯氧乙酸，在S早期促进端粒酶活性的增加，而生长抑制类似物2，3-二氯苯氧乙酸则没有这种作用。吲哚-3-乙酸对端粒酶活性的诱导作用呈剂量依赖性。综上所述，这些数据表明，烟草细胞中的端粒酶活性是以细胞周期依赖的方式调节的，并且生长素特异性地诱导了S期端粒酶活性的增加。
Telomerase activity was measured at each phase of the cell cycle in synchronized tobacco (Nicotiana tabacum) BY-2 cells in suspension culture with the use of the telomeric repeat amplification protocol assay. The activity was low or undetectable at most phases of the cell cycle but showed a marked increase at early S phase. The induction of telomerase activity was not affected by the S phase blockers aphidicolin (which inhibits DNA polymerase α) or hydroxyurea (which inhibits ribonucleotide reductase), but it was prevented by olomoucine, an inhibitor of Cdc2/Cdk2 kinases that blocks G1-S cell cycle transition. These results suggest that the induction of telomerase activity is not directly coupled to DNA replication by conventional DNA polymerases, but rather is triggered by the entry of cells into S phase. Various analogs of the plant hormone auxin, including indole-3-acetic acid, α-naphthaleneacetic acid, and 2,4-dichlorophenoxyacetic acid, potentiated the increase in telomerase activity at early S phase; the growth-inactive analog 2,3-dichlorophenoxyacetic acid, however, had no such effect. Potentiation by indole-3-acetic acid of the induction of telomerase activity was dose dependent. Together, these data indicate that telomerase activity in tobacco cells is regulated in a cell cycle-dependent manner, and that the increase in activity at S phase is specifically inducible by auxin.
登录
查看更多内容
无细胞系统中蛋白激酶 A 和 C 对丝裂原激活蛋白激酶激活的调节。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
VanRenterghem,B;Browning,MD;Maller,JL
通讯作者：
Maller,JL
DOI：
10.1073/pnas.93.25.14422
发表时间：
1996-12-10
影响因子：
11.1
作者：
Fitzgerald, MS;McKnight, TD;Shippen, DE
通讯作者：
Shippen, DE
影响因子：
56.9
作者：
KIM, NW;PIATYSZEK, MA;SHAY, JW
通讯作者：
SHAY, JW
DOI：
10.1002/j.1460-2075.1994.tb06620.x
发表时间：
1994-07
期刊：
The EMBO Journal
影响因子：
--
作者：
L. L. Mantell-L.;C. Greider
通讯作者：
L. L. Mantell-L.;C. Greider