Molecular dissection of Penelope transposable element regulatory machinery.
Molecular dissection of Penelope transposable element regulatory machinery.
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DOI：
10.1093/nar/gkm1166
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发表时间：
2008-05
影响因子：
14.9
通讯作者：
Evgen'ev M
中科院分区：
文献类型：
--
作者：
Schostak N;Pyatkov K;Zelentsova E;Arkhipova I;Shagin D;Shagina I;Mudrik E;Blintsov A;Clark I;Finnegan DJ;Evgen'ev M
penelope -样元件（PLEs）是一类新的逆转录元件，在至少10个动物门的80多个物种中被发现。从果蝇中分离出的Penelope是该纲中唯一已知的转位活性代表。虽然先前已经在D. virilis和D. melanogaster转基因菌株中描述了Penelope主要转录物的大小和结构，但Penelope调控区域的结构仍然未知。为了确定推测的Penelope启动子和增强子样元件的定位，将推测的Penelope调控区域的片段与CAT报告基因连接，并通过p -element介导的转化引入D. melanogaster。利用ELISA测定CAT表达水平和RNA研究（包括RT-PCR）得到的结果表明，活性Penelope转座子含有一个内部启动子，类似于LINEs的TATA-less启动子。结果还表明，部分Penelope调控序列通过提高其在卵巢中的表达而降低其在胴体中的表达来控制其在成虫卵巢中的优先表达。本文讨论了Penelope内含子对其功能和进化的可能意义，以及Penelope插入对邻近基因的影响。
Penelope-like elements (PLEs) represent a new class of retroelements identified in more than 80 species belonging to at least 10 animal phyla. Penelope isolated from Drosophila virilis is the only known transpositionally active representative of this class. Although the size and structure of the Penelope major transcript has been previously described in both D. virilis and D. melanogaster transgenic strains, the architecture of the Penelope regulatory region remains unknown. In order to determine the localization of presumptive Penelope promoter and enhancer-like elements, segments of the putative Penelope regulatory region were linked to a CAT reporter gene and introduced into D. melanogaster by P-element-mediated transformation. The results obtained using ELISA to measure CAT expression levels and RNA studies, including RT–PCR, suggest that the active Penelope transposon contains an internal promoter similar to the TATA-less promoters of LINEs. The results also suggest that some of the Penelope regulatory sequences control the preferential expression in the ovaries of the adult flies by enhancing expression in the ovary and reducing expression in the carcass. The possible significance of the intron within Penelope for the function and evolution of PLEs, and the effect of Penelope insertions on adjacent genes, are discussed.
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DOI：
10.1073/pnas.92.17.8050
发表时间：
1995-08-15
影响因子：
11.1
作者：
PETROV, DA;SCHUTZMAN, JL;LOZOVSKAYA, ER
通讯作者：
LOZOVSKAYA, ER
影响因子：
12.3
作者：
Ohler U;Liao GC;Niemann H;Rubin GM
通讯作者：
Rubin GM
影响因子：
3.3
作者：
Juneau, Kara;Miranda, Molly;Davis, Ronald W.
通讯作者：
Davis, Ronald W.
影响因子：
5.3
作者：
MINCHIOTTI, G;DINOCERA, PP
通讯作者：
DINOCERA, PP
影响因子：
4
作者：
Finnegan, David J.
通讯作者：
Finnegan, David J.