Cleaved intracellular plasminogen activator inhibitor 2 in human myeloleukaemia cells is a marker of apoptosis.
Cleaved intracellular plasminogen activator inhibitor 2 in human myeloleukaemia cells is a marker of apoptosis.
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DOI：
10.1038/bjc.1994.407
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发表时间：
1994-11
影响因子：
8.8
通讯作者：
Vintermyr, O. K.
中科院分区：
文献类型：
--
作者：
Jensen, P. H.;Cressey, L. I.;Gjertsen, B. T.;Madsen, P.;Mellgren, G.;Hokland, P.;Gliemann, J.;Doskeland, S. O.;Lanotte, M.;Vintermyr, O. K.
研究了磷酸酶抑制剂冈田酸和calyculin A以及蛋白质合成抑制剂放线菌酮对人早幼粒白血病细胞株NB 4凋亡过程中纤溶酶原激活物抑制剂2（派-2）的蛋白水解修饰。通过形态学和生物化学标准确定细胞死亡的突变类型。在细胞匀浆中，用[125 I]尿激酶纤溶酶原激活剂（uPA）探测派-2，并在还原十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和放射自显影后检测到十二烷基硫酸钠稳定的M（r）80，000复合物。在细胞凋亡过程中，始终检测到较小的（M（r）70，000）uPA-PAI-2复合物。修饰是在派-2部分，因为[125 I]uPA示踪剂可以以其完整形式从复合物中提取。因此，裂解的派-2同种型是早幼粒细胞NB 4细胞系中细胞凋亡的生化标志物。在从急性和慢性髓性白血病患者骨髓中吸出的纯化人单核白血病细胞制成的匀浆中也检测到经修饰的派-2亚型。
The proteolytic modification of plasminogen activator inhibitor 2 (PAI-2) was studied during apoptosis in the human promyelocytic leukaemic NB4 cell line during treatment with the phosphatase inhibitors okadaic acid and calyculin A as well as the protein synthesis inhibitor cycloheximide. The apoptic type of cell death was ascertained by morphological and biochemical criteria. In cell homogenates PAI-2 was probed by [125I]urokinase plasminogen activator (uPA) and detected as a sodium dodecyl sulphate-stable M(r) 80,000 complex after reducing sodium dodecyl sulphate-polyacrylamide gel electrophoresis and autoradiography. During apoptosis a smaller (M(r) 70,000) uPA-PAI-2 complex was consistently detected. The modification was in the PAI-2 moiety, as the [125I]uPA tracer could be extracted in its intact form from the complex. Thus the cleaved PAI-2 isoform is a biochemical marker of apoptosis in the promyelocytic NB4 cell line. The modified PAI-2 isoform was also detected in homogenates made from purified human mononuclear leukaemic cells aspirated from the bone marrow of patients suffering from acute and chronic myeloid leukaemia.
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DOI：
10.1091/mbc.1.13.1043
发表时间：
1990-12-01
期刊：
CELL REGULATION
影响因子：
--
作者：
JENSEN, PH;CHRISTENSEN, EI;ANDREASEN, PA
通讯作者：
ANDREASEN, PA
DOI：
10.1111/j.1432-1033.1993.tb17906.x
发表时间：
1993-05-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
JENSEN, PH;LORAND, L;GLIEMANN, J
通讯作者：
GLIEMANN, J
影响因子：
11.4
作者：
BELIN, D;WOHLWEND, A;VASSALLI, JD
通讯作者：
VASSALLI, JD
影响因子：
3.5
作者：
HOUGE, G;DOSKELAND, SO;LANOTTE, M
通讯作者：
LANOTTE, M
影响因子：
2.9
作者：
CHOMCZYNSKI, P;SACCHI, N
通讯作者：
SACCHI, N