IL‑33 restricts invasion and adhesion of trophoblast cell line JEG3 by downregulation of integrin α4β1 and CD62L.
IL‑33 restricts invasion and adhesion of trophoblast cell line JEG3 by downregulation of integrin α4β1 and CD62L.
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IL-33通过下调整合素α4β1和CD62L限制滋养层细胞系JEG3的侵袭和粘附
DOI：
10.3892/mmr.2017.7085
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发表时间：
2017-10
影响因子：
3.4
通讯作者：
Jin LP
中科院分区：
文献类型：
--
作者：
Wang XH;Liu W;Fan DX;Hu WT;Li MQ;Zhu XY;Jin LP
白细胞介素-33 （IL-33）通过下调E-cadherin的表达促进癌细胞的迁移。先前的研究表明，IL-33刺激滋养细胞的增殖。然而，IL-33对滋养细胞粘附和侵袭的影响尚未得到详细的研究。本研究采用免疫组织化学方法检测了妊娠早期妇女绒毛中IL-33及其受体IL-1受体样1 （ST2）的表达。流式细胞术证实了ST2在人滋养细胞和绒毛膜癌细胞JAR、BeWo、JEG3和HTR8上的表达。通过细胞粘附、Matrigel侵袭和流式细胞术分析重组人IL-33 （rhIL-33）对细胞粘附、侵袭及相关分子的影响。目前的研究发现人类滋养细胞表达IL-33和ST2。RhIL-33抑制滋养细胞侵袭和粘附，降低粘附和侵袭相关分子，如整合素α4β1和CD62L。因此，这些结果提示IL-33可能通过粘附和侵袭相关分子限制滋养层细胞的侵袭和着床，有助于妊娠早期胎盘的形成和正常妊娠的维持。
Interleukin-33 (IL-33) promotes migration of cancer cells through downregulating the expression of E-cadherin. Previous studies have demonstrated that IL-33 stimulates the proliferation of trophoblasts. However, the effect of IL-33 on the adhesion and invasion of trophoblasts has not been investigated in detail. In the present study, the expression of IL-33 and its receptor, IL-1 receptor-like 1 (ST2), was examined in villi from women during early pregnancy using immunohistochemistry. ST2 expression on human trophoblast and choriocarcinoma cell lines JAR, BeWo, JEG3 and HTR8 was confirmed by flow cytometry (FCM) assay. The effect of recombinant human IL-33 (rhIL-33) on adhesion, invasion and associated molecules was analyzed by cell adhesion, Matrigel invasion and FCM assays. The current study identified that human trophoblasts expressed IL-33 and ST2. RhIL-33 inhibited trophoblast invasion and adhesion, and decreased adhesion and invasion-associated molecules such as integrin α4β1 and CD62L. Therefore, these results suggest that IL-33 may serve an important role in limiting invasion and implantation of trophoblasts by adhesion and invasion-associated molecules, contributing to the formation of the placenta and maintenance of normal pregnancy during early pregnancy.
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DOI：
10.1530/ror.0.0020084
发表时间：
1997-05-01
期刊：
Reviews of reproduction
影响因子：
--
作者：
Aplin, J D
通讯作者：
Aplin, J D
影响因子：
5
作者：
Iikura, Motoyasu;Suto, Hajime;Nakae, Susumu
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Nakae, Susumu
影响因子：
3.8
作者：
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Moffett A
影响因子：
4.4
作者：
Fock, Valerie;Mairhofer, Mario;Pollheimer, Juergen
通讯作者：
Pollheimer, Juergen
DOI：
10.1161/atvbaha.111.231431
发表时间：
2011-09-01
影响因子：
8.7
作者：
Demyanets, Svitlana;Konya, Viktoria;Wojta, Johann
通讯作者：
Wojta, Johann