Collagen type IV at the fetal-maternal interface.
Collagen type IV at the fetal-maternal interface.
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DOI：
10.1016/j.placenta.2014.10.012
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发表时间：
2015-01
期刊：
影响因子：
3.8
通讯作者：
Moffett A
中科院分区：
文献类型：
--
作者：
Oefner CM;Sharkey A;Gardner L;Critchley H;Oyen M;Moffett A
细胞外基质蛋白在影响滋养细胞的侵袭中起着至关重要的作用。然而，胶原蛋白和IV型胶原蛋白（col-IV）在植入部位的作用尚不清楚。采用免疫组化方法测定月经周期和妊娠早期子宫内膜和蜕膜中ⅰ、ⅲ、ⅳ、ⅵ型胶原的分布。通过qPCR和免疫组化蛋白定位检测生殖周期中coli - iv α链的表达。用透射电镜观察胎盘中大肠杆菌的结构。最后，通过免疫组织化学定位coli - iv α链NC1结构域和胶原受体的表达。coliv α链在月经周期和去个体化过程中选择性上调。在体外和体内，原代上皮外滋养细胞表达胶原受体并分泌coli - iv，导致基底蜕膜中coli - iv水平高于顶蜕膜。在胎盘绒毛间质中发现了一种新的coli - iv的三维网络表达模式。已知coliv α链的NC1结构域可调节肿瘤细胞的迁移，并确定了这些结构域在基底蜕膜和顶蜕膜中的选择性表达。Col-IV在胎盘中以新的形式表达。这些发现表明，col-IV不仅代表了一种提供组织完整性的结构蛋白，而且还影响着床部位滋养细胞的侵袭行为。我们的数据表明黄体酮可能调节IV型胶原。原代上皮外滋养细胞分泌IV型胶原。我们提出了胎盘中IV型胶原的一个新的三维网络。滋养层细胞表达整合素α 10、整合素α 11和胶原受体DDR-1和DDR-2。
Extracellular matrix proteins play a crucial role in influencing the invasion of trophoblast cells. However the role of collagens and collagen type IV (col-IV) in particular at the implantation site is not clear. Immunohistochemistry was used to determine the distribution of collagen types I, III, IV and VI in endometrium and decidua during the menstrual cycle and the first trimester of pregnancy. Expression of col-IV alpha chains during the reproductive cycle was determined by qPCR and protein localisation by immunohistochemistry. The structure of col-IV in placenta was examined using transmission electron microscopy. Finally, the expression of col-IV alpha chain NC1 domains and collagen receptors was localised by immunohistochemistry. Col-IV alpha chains were selectively up-regulated during the menstrual cycle and decidualisation. Primary extravillous trophoblast cells express collagen receptors and secrete col-IV in vitro and in vivo, resulting in the increased levels found in decidua basalis compared to decidua parietalis. A novel expression pattern of col-IV in the mesenchyme of placental villi, as a three-dimensional network, was found. NC1 domains of col-IV alpha chains are known to regulate tumour cell migration and the selective expression of these domains in decidua basalis compared to decidua parietalis was determined. Col-IV is expressed as novel forms in the placenta. These findings suggest that col-IV not only represents a structural protein providing tissue integrity but also influences the invasive behaviour of trophoblast cells at the implantation site. Our data suggest that progesterone might regulate collagen type IV. Primary extravillous trophoblast cells secrete collagen type IV. A novel three-dimensional network of collagen type IV in placenta is presented. Trophoblast cells express integrin alpha 10, integrin alpha 11 and collagen receptors DDR-1 and DDR-2.
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影响因子：
3.8
作者：
Chen, CP;Aplin, JD
通讯作者：
Aplin, JD
影响因子：
7.3
作者：
Ball, E;Bulmer, JN;Robson, SC
通讯作者：
Robson, SC
影响因子：
3.8
作者：
APLIN, JD
通讯作者：
APLIN, JD
影响因子：
6.1
作者：
BURROWS, TD;KING, A;LOKE, YW
通讯作者：
LOKE, YW
影响因子：
4.4
作者：
CUTTING, GR;KAZAZIAN, HH;FRANCOMANO, CA
通讯作者：
FRANCOMANO, CA