Small intestinal metabolomics analysis reveals differentially regulated metabolite profiles in obese rats and with prebiotic supplementation.
Small intestinal metabolomics analysis reveals differentially regulated metabolite profiles in obese rats and with prebiotic supplementation.
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小肠代谢组学分析揭示了肥胖大鼠以及补充益生元后代谢物谱的差异调节情况。
DOI：
10.1007/s11306-022-01920-9
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发表时间：
2022-07-23
期刊：
影响因子：
3.6
通讯作者：
Duca, Frank A.
中科院分区：
文献类型：
--
作者：
Meyer, Rachel K.;Bime, Megan A.;Duca, Frank A.
肥胖的发生部分是由于高脂肪，高糖和低纤维饮食的消费，并与肠道微生物组的改变有关。益生元补充剂可以逆转肥胖并有益地改变肠道微生物组，这在啮齿动物的先前研究中得到了证明。然而，小肠代谢组在肥胖和益生元补充啮齿动物中的作用从未被研究过。研究和比较健康和肥胖大鼠以及补充益生元低聚果糖（OFS）的肥胖大鼠小肠代谢组。使用UPLC-MS/MS对健康的普通饲料喂养的、高脂肪饮食诱导的肥胖的和补充有低聚果糖的肥胖大鼠的小肠内容物进行非靶向代谢组学。使用UPLC-MS进行肝肠胆汁酸的定量，以确定肥胖和纤维补充对胆汁酸池组成的具体影响。肥胖大鼠的小肠代谢组与健康大鼠不同。补充OFS并没有显著改变小肠代谢组，但与肥胖大鼠相比，确实改变了几种代谢物的水平，包括胆汁酸代谢物、氨基酸代谢物和异源代谢物。此外，肥胖大鼠在肝肠循环中总胆汁酸较低，牛磺酸结合胆汁酸种类增加;在高脂肪喂养中补充OFS可逆转这种效应。肥胖与一个独特的小肠代谢组相关，OFS补充剂逆转了肥胖大鼠中改变的一些代谢物水平。未来对本研究中确定的特定代谢物的影响的研究将为纤维补充剂改善体重的机制提供更深入的了解。
Obesity occurs partly due to consumption of a high-fat, high-sugar and low fiber diet and is associated with an altered gut microbiome. Prebiotic supplementation can reverse obesity and beneficially alter the gut microbiome, evidenced by previous studies in rodents. However, the role of the small intestinal metabolome in obese and prebiotic supplemented rodents has never been investigated. To investigate and compare the small intestinal metabolome of healthy and obese rats, as well as obese rats supplemented with the prebiotic oligofructose (OFS). Untargeted metabolomics was performed on small intestinal contents of healthy chow-fed, high fat diet-induced obese, and obese rats supplemented with oligofructose using UPLC-MS/MS. Quantification of enterohepatic bile acids was performed with UPLC-MS to determine specific effects of obesity and fiber supplementation on the bile acid pool composition. The small intestinal metabolome of obese rats was distinct from healthy rats. OFS supplementation did not significantly alter the small intestinal metabolome but did alter levels of several metabolites compared to obese rats, including bile acid metabolites, amino acid metabolites, and xenobiotic metabolites. Further, obese rats had lower total bile acids and increased taurine-conjugated bile acid species in enterohepatic circulation; this effect was reversed with OFS supplementation in high fat-feeding. Obesity is associated with a distinct small intestinal metabolome, and OFS supplementation reverses some metabolite levels that were altered in obese rats. Future research into the effects of specific metabolites identified in this study will provide deeper insight into the mechanism of fiber supplementation on improved body weight.
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影响因子：
7.9
作者：
Abdel-Aziz, Heba;Wadie, Walaa;Khayyal, Mohamed T.
通讯作者：
Khayyal, Mohamed T.
影响因子：
6.9
作者：
Cluny, Nina L.;Eller, Lindsay K.;Sharkey, Keith A.
通讯作者：
Sharkey, Keith A.
影响因子：
4.6
作者：
Gonzalez-Blazquez, Raquel;Alcala, Martin;Somoza, Beatriz
通讯作者：
Somoza, Beatriz
影响因子：
15.1
作者：
de Cossio, Lourdes Fernandez;Fourrier, Celia;Castanon, Nathalie
通讯作者：
Castanon, Nathalie
影响因子：
24.5
作者：
Cani PD;Possemiers S;Van de Wiele T;Guiot Y;Everard A;Rottier O;Geurts L;Naslain D;Neyrinck A;Lambert DM;Muccioli GG;Delzenne NM
通讯作者：
Delzenne NM