Dstac Regulates Excitation-Contraction Coupling in Drosophila Body Wall Muscles.
Dstac Regulates Excitation-Contraction Coupling in Drosophila Body Wall Muscles.
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DOI：
10.3389/fphys.2020.573723
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发表时间：
2020
影响因子：
4
通讯作者：
Kuwada JY
中科院分区：
文献类型：
--
作者：
Hsu IU;Linsley JW;Reid LE;Hume RI;Leflein A;Kuwada JY
Stac3通过调节L型电压门控钙通道(Cav通道)来调节脊椎动物骨骼肌的兴奋收缩偶联(EC偶联)。最近，在果蝇中发现了一个类似STAC的基因，Dstac，并发现它在神经元和肌肉中都有表达。在这里，我们证明了Dstac和Dmca1D，果蝇L类型的Cav通道，是幼虫正常运动所必需的。用针对Dstac和Dmca1D的特异性抗体进行免疫标记，发现Dstac和Dmca1D在幼虫的体壁肌肉中表达。此外，对Dstac和Dmca1D缺乏的幼虫肌肉进行的钙成像发现，Dstac和Dmca1D是兴奋收缩偶联所必需的。最后，Dstac似乎是在体壁肌肉中正常表达Dmca1D所必需的。这些结果表明，Dstac在EC偶联过程中调节Dmca1D，从而调节肌肉收缩。
Stac3 regulates excitation-contraction coupling (EC coupling) in vertebrate skeletal muscles by regulating the L-type voltage-gated calcium channel (Cav channel). Recently a stac-like gene, Dstac, was identified in Drosophila and found to be expressed by both a subset of neurons and muscles. Here, we show that Dstac and Dmca1D, the Drosophila L-type Cav channel, are necessary for normal locomotion by larvae. Immunolabeling with specific antibodies against Dstac and Dmca1D found that Dstac and Dmca1D are expressed by larval body-wall muscles. Furthermore, Ca2+ imaging of muscles of Dstac and Dmca1D deficient larvae found that Dstac and Dmca1D are required for excitation-contraction coupling. Finally, Dstac appears to be required for normal expression levels of Dmca1D in body-wall muscles. These results suggest that Dstac regulates Dmca1D during EC coupling and thus muscle contraction.
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影响因子：
16.6
作者：
通讯作者：
--
DOI：
10.1007/s00018-017-2478-0
发表时间：
2017-07
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Salzer U;Kostan J;Djinović-Carugo K
通讯作者：
Djinović-Carugo K
DOI：
10.1073/pnas.1612441113
发表时间：
2016-09-27
影响因子：
11.1
作者：
Polster, Alexander;Nelson, Benjamin R.;Beam, Kurt G.
通讯作者：
Beam, Kurt G.
影响因子：
64.8
作者：
SCHNEIDER, MF;CHANDLER, WK
通讯作者：
CHANDLER, WK
DOI：
10.1073/pnas.1619238114
发表时间：
2017-01-10
影响因子：
11.1
作者：
Linsley, Jeremy W.;Hsu, I-Uen;Kuwada, John Y.
通讯作者：
Kuwada, John Y.