Ultrarobust Biochips with Metal-Organic Framework Coating for Point-of-Care Diagnosis.
Ultrarobust Biochips with Metal-Organic Framework Coating for Point-of-Care Diagnosis.
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DOI：
10.1021/acssensors.7b00762
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发表时间：
2018-02-23
期刊：
影响因子：
8.9
通讯作者：
Singamaneni S
中科院分区：
文献类型：
--
作者：
Wang C;Wang L;Tadepalli S;Morrissey JJ;Kharasch ED;Naik RR;Singamaneni S
大多数依靠抗体作为识别元件的生物传感器会因抗体变性而在高温、有机溶剂或蛋白酶等恶劣环境条件下失效，并且需要严格的储存条件和规定的保质期，从而限制了它们在护理点和资源有限环境中的应用。在这里，利用金属有机框架（MOF）封装来保留与纳米转导器缀合的抗体的生物功能。本研究研究了 MOF 涂层的几个参数（包括生长时间、表面形态、厚度和前体浓度），这些参数决定了在干燥和潮湿环境中针对不同蛋白质变性条件的保存功效。基于金纳米棒作为纳米传感器的等离子体生物传感器被用作模型生物诊断平台。通过 MOF 封装获得的保存功效与其他两种常用材料（蔗糖和丝素蛋白）进行了比较。结果表明，MOF 涂层在高温（80 °C）、二甲基甲酰胺和蛋白酶溶液等多种恶劣条件下优于蔗糖和丝素蛋白涂层，因为 MOF 完全封装、在潮湿环境中稳定且易于在使用时去除。我们相信这项研究将扩大这种通用方法的适用性，用于保存不同类型的片上生物诊断试剂和生物传感器/生物测定，从而在资源有限的环境中扩大先进诊断技术的优势。
Most biosensors relying on antibodies as recognition elements fail in harsh environment conditions such as elevated temperatures, organic solvents or proteases because of antibody denaturation, and require strict storage conditions with defined shelf-life, thus limiting their applications in point-of-care and resource-limited settings. Here, a metal-organic framework (MOF) encapsulation is utilized to preserve the biofunctionality of antibodies conjugated to nanotransducers. This study investigates several parameters of MOF coating (including growth time, surface morphology, thickness and precursor concentrations) that determine the preservation efficacy against different protein denaturing conditions in both dry and wet environments. A plasmonic biosensor based on gold nanorods as the nanotransducers is employed as a model biodiagnostic platform. The preservation efficacy attained through MOF encapsulation is compared to two other commonly employed materials (sucrose and silk fibroin). The results show that MOF coating outperforms sucrose and silk fibroin coatings under several harsh conditions including high temperature (80 °C), dimethylformamide and protease solution, owing to complete encapsulation, stability in wet environment and ease of removal at point-of-use by the MOF. We believe this study will broaden the applicability of this universal approach for preserving different types of on-chip biodiagnostic reagents and biosensors/bioassays, thus extending the benefits of advanced diagnostic technologies in resource-limited settings.
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