Long-term maintenance of polysaccharide-specific antibodies by IgM-secreting cells.
Long-term maintenance of polysaccharide-specific antibodies by IgM-secreting cells.
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DOI：
10.4049/jimmunol.1100783
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Kearney JF
中科院分区：
文献类型：
--
作者：
Foote JB;Mahmoud TI;Vale AM;Kearney JF
许多细菌相关多糖可诱导长效抗体反应，从而抵御病原微生物。多糖特异性抗体滴度的维持可能是由于长寿命的浆细胞或由于多糖持久性而持续的抗原驱动的 B 细胞激活。 BALB/c 和 VHJ558.3 转基因 (TG) 小鼠对 α 1→3-葡聚糖 (DEX) 的反应是在 7 天时产生峰值抗 DEX 反应，然后维持血清抗体水平长达 150 天。对 DEX 的细胞反应分析发现，脾脏中有一群短命的、环磷酰胺敏感的 DEX 特异性浆母细胞，而骨髓中有一群静止的、环磷酰胺抗性的 DEX 特异性抗体分泌细胞。 BrdU 脉冲追踪实验证明了骨髓中 DEX 特异性抗体分泌群体的寿命。免疫后 90 天，脾脏 DEX 特异性浆母细胞位于红髓细胞中，并具有持续存在的 DEX 相关 CD11c+ 树突状细胞，而 28 天后在骨髓中未检测到 DEX。使用环磷酰胺和抗 CD20 治疗选择性消除短命 DEX 特异性浆母细胞和记忆 B1b B 细胞，对血清抗 DEX 抗体的维持影响极小。总的来说，这些发现表明，血清多糖特异性抗体的维持是脾脏中持续抗原驱动的短寿命浆母细胞形成和骨髓中长期维持的静态抗体分泌细胞群的结果。
Many bacteria-associated polysaccharides induce long-lived antibody responses that protect against pathogenic microorganisms. The maintenance of polysaccharide-specific antibody titers may be due to long-lived plasma cells or ongoing antigen-driven B cell activation due to polysaccharide persistence. BALB/c and VHJ558.3 transgenic (TG) mice respond to α 1→3-dextran (DEX) by generating a peak anti-DEX response at 7 days, followed by maintenance of serum antibody levels for up to 150 days. Analysis of the cellular response to DEX identified a population of short-lived, cyclophosphamide sensitive DEX-specific plasmablasts in the spleen, and a quiescent, cyclophosphamide resistant DEX-specific antibody-secreting population in the bone marrow. BrdU pulse-chase experiments demonstrated the longevity of the DEX-specific antibody-secreting population in the bone marrow. Splenic DEX-specific plasmablasts were located in the red pulp with persisting DEX-associated CD11c+ dendritic cells 90 days after immunization, whereas DEX was not detected in the bone marrow after 28 days. Selective depletion of short-lived DEX-specific plasmablasts and memory B1b B cells using cyclophosphamide and anti-CD20 treatment had a minimal impact on the maintenance of serum anti-DEX antibodies. Collectively, these findings demonstrate that the maintenance of serum polysaccharide-specific antibodies is the result of continuous antigen-driven formation of short-lived plasmablasts in the spleen and a quiescent population of antibody-secreting cells maintained in the bone marrow for a long duration.
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影响因子：
4.4
作者：
Hauser, AE;Debes, GF;Manz, RA
通讯作者：
Manz, RA
影响因子：
64.8
作者：
Crotty, S;Kersh, EN;Ahmed, R
通讯作者：
Ahmed, R
影响因子：
20.3
作者：
Ellyard, JI;Avery, DT;Tangye, SG
通讯作者：
Tangye, SG
DOI：
10.1084/jem.20080979
发表时间：
2008-12-22
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Choi YS;Baumgarth N
通讯作者：
Baumgarth N
影响因子：
6.4
作者：
Grinsell, M;Weinhold, LC;Kozel, TR
通讯作者：
Kozel, TR