Influence of Selenium on the Growth of N-Nitrosomethylurea-Induced Mammary Tumor Cells in Culture 1
Influence of Selenium on the Growth of N-Nitrosomethylurea-Induced Mammary Tumor Cells in Culture 1
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硒对 N-亚硝基甲基脲诱导的乳腺癌细胞培养生长的影响 1
DOI：
10.3181/00379727-180-42139
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发表时间：
1985
影响因子：
--
通讯作者：
K. McConnell
中科院分区：
文献类型：
--
作者：
W. Lewko;K. McConnell
Abstract Selenium is an essential dietary trace element which has anticancer properties. Among its effects in rats, selenium has been shown to inhibit the development of carcinogen-induced mammary tumors by interfering with the postinitiation, promotion phase of carcinogenesis. We studied the effects of selenium on the growth of rat mammary tumor cells in primary culture. Our objective was to determine whether selenium had any direct influence on cell growth which might explain its influence on tumor development. Rat mammary tumors were induced by N-nitrosomethylurea. Tumor epithelium was prepared by collagenase dispersion and the cells were separated by Ficoll gradient centrifugation. The tumor epithelium was grown in primary culture using a defined serum-free medium. The addition of low concentrations of sodium selenite, less than 1.0 μg/ml, stimulated tumor cell proliferation. Protein synthesis and the production of type IV collagen increased within the first hour of exposure, prior to any measurable increase in DNA synthesis. Concentrations of selenite greater than 1.0 μg/ml inhibited cell proliferation, the synthesis of protein, and the replication of DNA in a dose-related manner. These studies demonstrated that selenium has the potential to influence the postinitiation phase of rat mammary tumorigenesis by directly altering the growth of tumor cells, possibly through the regulation of protein synthesis.
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影响因子：
4.7
作者：
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影响因子：
11.2
作者：
Ip,C
通讯作者：
Ip,C
在化学致癌作用的起始和促进阶段通过补充硒来预防乳腺肿瘤。
影响因子：
11.2
作者：
Ip,C
通讯作者：
Ip,C
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影响因子：
9.7
作者：
Chatterjee,M;Banerjee,MR
通讯作者：
Banerjee,MR
硒和小鼠乳腺肿瘤发生：可能机制的研究。
影响因子：
11.2
作者：
Medina,D;Lane,HW;Tracey,CM
通讯作者：
Tracey,CM