Multiple single-cell genomes provide insight into functions of uncultured Deltaproteobacteria in the human oral cavity.
Multiple single-cell genomes provide insight into functions of uncultured Deltaproteobacteria in the human oral cavity.
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DOI：
10.1371/journal.pone.0059361
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Podar M
中科院分区：
文献类型：
--
作者：
Campbell AG;Campbell JH;Schwientek P;Woyke T;Sczyrba A;Allman S;Beall CJ;Griffen A;Leys E;Podar M
尽管研究历史悠久，但许多与人类口腔相关的细菌尚未被培养。将未培养物种的存在或丰富度与口腔健康或疾病联系起来的研究强调了这些群落成员的重要性。因此，我们对来自 Desulfobulbus 和 Desulfovibrio（Deltaproteobacteria 类）的几个单细胞基因组扩增子进行了测序，以更好地了解它们在人类口腔群落中的功能及其与牙周炎以及其他全身性疾病的关系。将口腔 Desulfobulbus 和 Desulfovibrio 物种的基因组数据与其他可用的 deltaproteobacterial 基因组（包括宿主相关物种的子集）进行比较。虽然这两个群体与其他环境三角洲变形菌基因组共享大量基因，但它们编码大量独特的基因，这些基因似乎在宿主环境中的生存中发挥作用。其中许多基因与已知人类病原体的毒力和宿主适应因子相似，表明口腔三角洲变形菌有可能在牙周病的病因学中发挥作用。
Despite a long history of investigation, many bacteria associated with the human oral cavity have yet to be cultured. Studies that correlate the presence or abundance of uncultured species with oral health or disease highlight the importance of these community members. Thus, we sequenced several single-cell genomic amplicons from Desulfobulbus and Desulfovibrio (class Deltaproteobacteria) to better understand their function within the human oral community and their association with periodontitis, as well as other systemic diseases. Genomic data from oral Desulfobulbus and Desulfovibrio species were compared to other available deltaproteobacterial genomes, including from a subset of host-associated species. While both groups share a large number of genes with other environmental Deltaproteobacteria genomes, they encode a wide array of unique genes that appear to function in survival in a host environment. Many of these genes are similar to virulence and host adaptation factors of known human pathogens, suggesting that the oral Deltaproteobacteria have the potential to play a role in the etiology of periodontal disease.
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影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
14.9
作者：
Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A
影响因子：
11
作者：
Griffen, Ann L.;Beall, Clifford J.;Leys, Eugene J.
通讯作者：
Leys, Eugene J.
影响因子：
4.4
作者：
Fernández, L;Márquez, I;Guijarro, JA
通讯作者：
Guijarro, JA
影响因子：
3.8
作者：
Fuller, TE;Kennedy, MJ;Lowery, DE
通讯作者：
Lowery, DE