Physiologic properties of Müller cells from human eyes affected with uveal melanoma.
Physiologic properties of Müller cells from human eyes affected with uveal melanoma.
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受葡萄膜黑色素瘤影响的人眼 Müller 细胞的生理特性
DOI：
10.1167/iovs.12-9746
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发表时间：
2012
影响因子：
4.4
通讯作者：
Bringmann A
中科院分区：
文献类型：
--
作者：
Grosche A;Pannicke T;Karl A;Iandiev I;Francke M;Wiedemann P;Reichenbach A;Bringmann A
目的：研究来自健康和病理改变眼睛的人类穆勒细胞的生理特征。方法：使用来自器官捐献者和葡萄膜黑色素瘤患者的人体组织。几只患有黑色素瘤的眼睛也出现视网膜脱离。将新鲜制备的切片与商业活性染料一起孵育，优先染色穆勒细胞。通过记录细胞体的横截面积来观察穆勒细胞对低渗应激的反应。在全细胞膜片钳实验中并行研究了电生理特性。结果：与健康对照相比，葡萄膜黑色素瘤眼的 Müller 细胞中由内向整流 Kir 通道介导的内向 K+ 电流显着降低。这伴随着膜电位的降低。这两种效应在黑色素瘤导致广泛视网膜脱离的眼睛细胞中更为强烈。低渗溶液的应用不会影响健康器官捐献者的穆勒细胞。相比之下，一些黑色素瘤眼睛的米勒细胞响应低渗溶液而增加了体细胞大小。这种效应在来自广泛视网膜脱离的眼睛的细胞中更为严重。炎症介质花生四烯酸可引起米勒细胞肿胀，而抗炎物质可减轻肿胀。结论：人体组织实验证实了视网膜病理动物模型中关于反应性米勒细胞典型变化的早期数据。低渗应激优先诱导受黑色素瘤影响的眼睛细胞中的穆勒细胞肿胀，这些细胞表现出内向电流幅度降低。广泛的黑色素瘤相关视网膜脱离增强了穆勒细胞的病理改变。
Purpose.: To study physiologic characteristics of human Müller cells from healthy and pathologically altered eyes.Methods.: Human tissue was used from organ donors and from patients affected with uveal melanoma. Several melanoma eyes also showed retinal detachment. Incubation of freshly prepared slices with a commercial vital dye preferentially stained Müller cells. The Müller cell response to hypotonic stress was observed by recording the cross-sectional area of cell somata. Electrophysiologic properties were investigated in parallel in whole-cell patch-clamp experiments.Results.: Inward K+ currents mediated by inwardly rectifying Kir channels were significantly decreased in Müller cells from eyes with uveal melanoma compared with healthy controls. This was accompanied by a decrease of the membrane potential. Both effects were stronger in cells from eyes where the melanoma had caused a widespread retinal detachment. Application of a hypotonic solution did not affect Müller cells from healthy organ donors. By contrast, Müller cells from some melanoma eyes increased their soma size in response to hypotonic solution. This effect was aggravated in cells from eyes with widespread retinal detachment. The inflammatory mediator, arachidonic acid, could induce Müller cell swelling, whereas anti-inflammatory substances reduced the swelling.Conclusions.: The experiments with human tissue confirm earlier data from animal models for retinal pathologies about typical alterations of reactive Müller cells. Hypotonic stress induced Müller cell swelling preferentially in cells from melanoma-affected eyes that displayed decreased inward current amplitudes. Widespread melanoma-associated retinal detachment potentiated the pathologic alterations of Müller cells.
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影响因子：
4.4
作者：
R. Linsenmeier;L. Padnick-Silver
通讯作者：
R. Linsenmeier;L. Padnick-Silver
胶质细胞衍生的谷氨酸介导视网膜自分泌细胞体积调节：VEGF 的激活
影响因子：
4.7
作者：
A. Wurm;T. Pannicke;P. Wiedemann;A. Reichenbach;A. Bringmann
通讯作者：
A. Bringmann
人眼光性视网膜病变的病理学发现。
DOI：
--
发表时间：
1991
期刊：
American journal of ophthalmology-glaucoma
影响因子：
--
作者：
W. Green;D. Robertson
通讯作者：
D. Robertson
视网膜整体中 Müller 胶质细胞的活体染料选择性染色
影响因子：
6.2
作者：
Ortrud Uckermann;I. Iandiev;M. Francke;K. Franze;J. Grosche;S. Wolf;L. Kohen;P. Wiedemann;A. Reichenbach;A. Bringmann
通讯作者：
A. Bringmann
膜电导的变化在视网膜脱离的渗透性神经胶质细胞肿胀中发挥致病作用。
影响因子：
6
作者：
A. Wurm;T. Pannicke;I. Iandiev;E. Bühner;U. Pietsch;A. Reichenbach;P. Wiedemann;S. Uhlmann;A. Bringmann
通讯作者：
A. Bringmann