Temporal depolarization of mitochondria during M phase.
Temporal depolarization of mitochondria during M phase.
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DOI：
10.1038/s41598-017-15907-3
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发表时间：
2017-11-22
影响因子：
4.6
通讯作者：
Ohta Y
中科院分区：
文献类型：
--
作者：
Hirusaki K;Yokoyama K;Cho K;Ohta Y
细胞中的线粒体活性必须受到严格控制，以响应细胞内环境的变化。尽管细胞内条件和线粒体形态发生了剧烈变化，但尚不清楚在细胞周期的M期期间如何控制线粒体活性。在这里，我们表明，线粒体活性在M期急剧变化。线粒体膜电位在M期进程中发生变化。线粒体的极化程度与间期细胞的线粒体相同，但在末期和胞质分裂前后去极化。胞质分裂后，线粒体膜电位恢复。此外，线粒体中超氧阴离子的产生显着减少中期，即使在存在的抗霉素A，复合物III的抑制剂。这些结果表明，在M期线粒体电子传递链的电子供应受到抑制。这种抑制可能减少了M期线粒体断裂产生的活性氧。
Mitochondrial activity in cells must be tightly controlled in response to changes in intracellular circumstances. Despite drastic changes in intracellular conditions and mitochondrial morphology, it is not clear how mitochondrial activity is controlled during M phase of the cell cycle. Here, we show that mitochondrial activity is drastically changed during M phase. Mitochondrial membrane potential changed during M phase progression. Mitochondria were polarized until metaphase to the same extent as mitochondria in interphase cells, but were depolarized at around telophase and cytokinesis. After cytokinesis, mitochondrial membrane potential was recovered. In addition, the generation of superoxide anions in mitochondria was significantly reduced at metaphase even in the presence of antimycin A, an inhibitor of complex III. These results suggest that the electron supply to the mitochondrial electron transfer chain is suppressed during M phase. This suppression might decrease the reactive oxygen species generated by the fragmentation of mitochondria during M phase.
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影响因子：
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作者：
Reczek CR;Chandel NS
通讯作者：
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