Altered Expression of D1 and D2 Dopamine Receptors in Vagal Neurons Innervating the Gastric Muscularis Externa in a Parkinson's Disease Rat Model.
Altered Expression of D1 and D2 Dopamine Receptors in Vagal Neurons Innervating the Gastric Muscularis Externa in a Parkinson's Disease Rat Model.
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帕金森病大鼠模型中支配胃外肌层的迷走神经元中 D1 和 D2 多巴胺受体的表达发生改变。
DOI：
10.3233/jpd-160817
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Zhu Jin-Xia
中科院分区：
文献类型：
--
作者：
Wang Zhi-Yong;Lian Hui;Zhou Li;Zhang Yi-Min;Cai Qing-Qing;Zheng Li-Fei;Zhu Jin-Xia
背景：帕金森病（PD）的特征是黑质（SN）中多巴胺能神经元变性，并伴有迷走神经背侧运动核（DMV）的病理改变。胃轻瘫是患者和动物模型中帕金森病常见的非运动系统症状。然而，这种症状的潜在机制尚不清楚。我们先前报道了在帕金森病动物模型的DMV中酪氨酸羟化酶（TH）表达增强以及胆碱乙酰转移酶（ChAT）表达降低，并且TH和ChAT与多巴胺受体D1和D2共定位。我们假设这些受体可能导致帕金森病中观察到的胃排空延迟。
目的：研究D1和D2在投射到胃的DMV神经元中的分布以及在帕金森病大鼠模型中其分布的改变。
方法：采用逆行追踪、双标记免疫荧光技术和蛋白质印迹法。
结果：将逆行示踪剂氟金（FG）注射到胃壁后，在DMV的尾侧和吻侧部分观察到FG标记的投射到胃的运动神经元，并且具有D1 -、D2 -和ChAT -免疫反应性（IR）的神经元在DMV中广泛共定位。在D1 -和D2 -IR神经元周围观察到许多TH - IR纤维。此外，在黑质双侧微量注射6 - 羟基多巴胺（6 - OHDA）的大鼠中，观察到DMV中D1表达降低和D2表达增强。
结论：结果表明多巴胺受体可能影响DMV中投射到胃的神经元的活性，其表达的改变可能导致帕金森病中观察到的胃轻瘫。
Background:Parkinson’s disease (PD) is characterized by dopaminergic neuron degeneration in the substantia nigra (SN).accompanied by pathology of the dorsal motor nucleus of the vagus (DMV). Gastroparesis is a common non-motor system.symptom of PD in patients and in animal models. However, the underlying mechanism of this symptom is not clear. We.previously reported on the expression of enhanced tyrosine hydroxylase (TH) and decreased choline acetyltransferase (ChAT).in the DMV of a PD animal model and colocalization of TH and ChAT with the dopamine receptors D1and D2. We hypothesize.that these receptors might contribute to the delayed gastric emptying observed in PD..Objective:To investigate the distribution of D1and D2in gastric-projecting DMV neurons and alteration of their distribution.in a PD rat model..Methods:Retrograde tracing, double-labeling immunofluorescence techniques and western blotting were used..Results:After injection of the retrograde tracer fluoro-gold (FG) into the gastric wall, FG-labeled gastric-projecting motoneurons were observed in the caudal and rostral parts of the DMV, and neurons with D1-, D2- and ChAT- immunoreactivity (IR).were widely colocalized in the DMV. Many TH-IR fibers were observed around the D1- and D2-IR neurons. Moreover,.decreased D1and enhanced D2expression in the DMV was observed in 6-hydroxydopamine (6-OHDA) rats that were treated.with a bilateral microinjection of 6-OHDA in the SN..Conclusions:The results indicate that dopamine receptors might affect the activity of gastric-projecting neurons in the DMV,.their altered expression may contribute to the gastroparesis observed in PD.
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影响因子：
5
作者：
Kalaitzakis, M. E.;Graeber, M. B.;Pearce, R. K. B.
通讯作者：
Pearce, R. K. B.
DOI：
10.1152/ajpgi.00527.2006
发表时间：
2007-04
期刊：
American journal of physiology. Gastrointestinal and liver physiology
影响因子：
--
作者：
Zhongling Zheng;R. Travagli
通讯作者：
Zhongling Zheng;R. Travagli
大鼠迷走神经背运动核多巴胺受体 D1 和 D2 免疫反应神经元的分布
影响因子：
2.7
作者：
Cai, Qing-Qing;Zheng, Li-Fei;Zhu, Jin-Xia
通讯作者：
Zhu, Jin-Xia
影响因子：
2.8
作者：
D. Armstrong;L. Manley;J. Haycock;L. Hersh
通讯作者：
D. Armstrong;L. Manley;J. Haycock;L. Hersh
6-OHDA 诱导的帕金森病大鼠模型舌下核胆碱能运动神经元中多巴胺受体表达的改变
影响因子：
3.1
作者：
Zhou L;Wang ZY;Lian H;Song HY;Zhang YM;Zhang XL;Fan RF;Zheng LF;Zhu JX
通讯作者：
Zhu JX