The human nasal microbiota and Staphylococcus aureus carriage.
The human nasal microbiota and Staphylococcus aureus carriage.
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DOI：
10.1371/journal.pone.0010598
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发表时间：
2010-05-17
期刊：
影响因子：
3.7
通讯作者：
Pace NR
中科院分区：
文献类型：
--
作者：
Frank DN;Feazel LM;Bessesen MT;Price CS;Janoff EN;Pace NR
金黄色葡萄球菌在人体内的定植是随后皮肤、血液、肺、心脏和其他深部组织临床感染的关键先决条件。S.金黄色葡萄球菌持续地或间歇地定殖于约50%的健康成年人的鼻孔，而约50%的一般人群很少或从不被该病原体定殖。因为鼻腔内的微生物聚生体可能是S.金黄色葡萄球菌定殖，我们确定了鼻腔微生物群的组成和动力学，并将特定微生物与S.金黄色葡萄球菌定植。从5名健康成人和26名住院患者的横断面上纵向收集鼻标本。金黄色葡萄球菌携带者和16名非携带者）。16S rRNA序列的非培养物依赖性分析揭示，健康受试者的鼻微生物群主要由放线菌门的成员组成（例如，丙酸杆菌属和棒状杆菌属），其他门的代表性比例较低，包括厚壁菌门（例如，葡萄球菌属）和变形菌门（例如肠杆菌属）。相比之下，住院患者鼻内微生物菌群富含S。金黄色葡萄球菌或表皮葡萄球菌，并且在几个放线菌群中减少，最明显的是痤疮丙酸杆菌。此外，在住院人群S.金黄色葡萄球菌定植与几种微生物类群的丰度呈负相关，包括S.表皮（p = 0.004）。 鼻孔环境由暂时稳定的微生物群定殖，其与珠被的其他区域不同。与S.金黄色葡萄球菌、epidermidis和其他群体表明，在鼻孔定植期间存在微生物竞争，这一发现可用于限制S.金黄色葡萄球菌定植。
Colonization of humans with Staphylococcus aureus is a critical prerequisite of subsequent clinical infection of the skin, blood, lung, heart and other deep tissues. S. aureus persistently or intermittently colonizes the nares of ∼50% of healthy adults, whereas ∼50% of the general population is rarely or never colonized by this pathogen. Because microbial consortia within the nasal cavity may be an important determinant of S. aureus colonization we determined the composition and dynamics of the nasal microbiota and correlated specific microorganisms with S. aureus colonization. Nasal specimens were collected longitudinally from five healthy adults and a cross-section of hospitalized patients (26 S. aureus carriers and 16 non-carriers). Culture-independent analysis of 16S rRNA sequences revealed that the nasal microbiota of healthy subjects consists primarily of members of the phylum Actinobacteria (e.g., Propionibacterium spp. and Corynebacterium spp.), with proportionally less representation of other phyla, including Firmicutes (e.g., Staphylococcus spp.) and Proteobacteria (e.g. Enterobacter spp). In contrast, inpatient nasal microbiotas were enriched in S. aureus or Staphylococcus epidermidis and diminished in several actinobacterial groups, most notably Propionibacterium acnes. Moreover, within the inpatient population S. aureus colonization was negatively correlated with the abundances of several microbial groups, including S. epidermidis (p = 0.004). The nares environment is colonized by a temporally stable microbiota that is distinct from other regions of the integument. Negative association between S. aureus, S. epidermidis, and other groups suggests microbial competition during colonization of the nares, a finding that could be exploited to limit S. aureus colonization.
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影响因子：
11.8
作者：
Davis, KA;Stewart, JJ;Hospenthal, DR
通讯作者：
Hospenthal, DR
DOI：
10.1073/pnas.0807920105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Fierer, Noah;Hamady, Micah;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
2.9
作者：
Frank, Daniel N.;Wysocki, Annette;Trent, Jonathan D.
通讯作者：
Trent, Jonathan D.
影响因子：
3.7
作者：
CHAO, A;LEE, SM
通讯作者：
LEE, SM
影响因子：
14.6
作者：
Gilbert, Mark;MacDonald, Judy;Conly, John
通讯作者：
Conly, John